N LINKED OLIGOSACCHARIDE PROFILING

通过对样品进行单糖组成分析 制备甲基葡萄糖苷的TMS衍生物 GC-MS分析。样品被酸解，然后进行 甲烷化后与吡啶和乙酸乙酯进行N-乙酰化反应 酸酐(用于检测氨基糖)。然后，样本被 用Tri-sil治疗。用气相色谱-质谱法分析了TMS中的甲基糖苷。 在Hewlett-Packard 5890 GC和5970 MSD上使用 30米DB1熔融石英毛细管柱。样本是 使用Hakomori甲基化进行糖基连锁分析 然后进行GC-MS联用分析。然后，样本被 用2M三氟乙酸酸进行酸解，效果 所有糖苷键的断裂。糖基残基为 用NaBD4还原，然后用乙酸乙酰化 酸酐/吡啶。由此产生的部分甲基化的阿迪醇 醋酸盐在30米长的Supelco 2330熔融硅胶柱上进行分析 用GC-MS联用惠普5890色谱柱和5970色谱柱 MSD。

The samples were analyzed for monosaccharide composition by preparing the TMS derivatives of the methylglycosides followed by GC-MS analysis. The samples were acid hydrolyzed then subjected to methanolysis followed by N-acetylation with pyridine and acetic anhydride (for detection of amino sugars). The samples were then treated with Tri-Sil. GC-MS analysis of the TMS methylglycosides was performed on a Hewlett-Packard 5890 GC coupled to a 5970 MSD using a 30-meter DB1 fused silica capillary column. The samples were subjected to glycosyl-linkage analysis using the Hakomori methylation procedure followed by combined GC-MS analysis. The samples were then subjected to acid hydrolysis using 2 M trifluoroacetic acid to effect cleavage of all glycosidic linkages. The glycosyl residues were reduced with NaBD4 and then acetylated using acetic anhydride/pyridine. The resulting partially methylated alditol acetates were analyzed on a 30-meter Supelco 2330 fused silica capil lary column by GC-MS using a Hewlett-Packard 5890 GC coupled to a 5970 MSD.