CORE--SCIENTIFIC

核心部分的目标有三个：(1)提供 对方案参与者的行政支持；(2)建立 化学分析和寡聚脱氧核苷酸的集中设施 综合；以及(3)举办访问科学家的系列研讨会 将被邀请与计划成员讨论他们的研究。 该方案的行政结构基本保持不变。这个 核心项目已扩大到包括尖端质谱仪 分析以及自动化DNA合成和纯化。质量 光谱分析将在加合物的分析中发挥关键作用 合成中间体；我们希望增加一个重要的新成分，LC/MS 热喷雾系统，到我们现有的设施。 寡核苷酸的合成对所有研究人员来说都是必不可少的 该计划；这可以通过集中的 服务设施。一种化学改性化合物的合成 寡聚脱氧核苷酸需要大规模制备修饰 脱氧核苷亚磷酰胺。这将在核心进行 设施。

The objectives of the core component are three-fold: (1) to provide administrative support for participants in the Program; (2) to establish centralized facilities for chemical analysis and oligodeoxynucleotide synthesis; and (3) to conduct a seminar series in which visiting scientists will be invited to discuss their research with Program members. The administrative structure of the Program is substantially unchanged. The Core Project has been expanded to include sophisticated mass spectrometer analysis as well as automated DNA synthesis and purification. Mass spectrometry will play a critical role in the analysis of adducts and synthetic intermediates; we hope to add an important new component, a LC/MS thermospray system, to our current facilities. Synthesis of oligodeoxynucleotides is essential for all investigators in the Program; this can most effectively be accomplished by a centralized service facility. Synthesis of certain chemically-modified oligodeoxynucleotides will require large scale preparation of modified deoxynucleoside phosphoramidites. This will be conducted in the core facility.