MULTIPLEX IMMUNOASSAY FOR ANTIBODIES IN MACAQUES

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。目的：为了保持健康的非人类灵长类动物用于生物医学研究，在大多数灵长类动物设施中对动物进行几种感染性病原体的常规筛查。在目前的实践中，猴子的血清样本是通过传统的免疫分析方法进行检测的，如酶联免疫吸附试验(ELISA)或Western印迹法，以检测特定的抗体。为了测试每个样本中针对多种试剂的抗体，传统的免疫分析方法既费时又费力。其目的是开发更有效的免疫分析方法，用于非人类灵长类动物的血清监测。因此，我们开发了一种新的多重血清诊断系统，其中可单独识别的荧光微球被纯化的完整病毒抗原包裹。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Objective: To maintain healthy nonhuman primates for use in biomedical research, animals are routinely screened at most primate facilities for several infectious agents. In current practice, monkey serum samples are tested by conventional immunoassays such as enzyme linked immunosorbent assay (ELISA) or Western blot for the detection of specific antibodies. For testing antibodies against multiple agents in each sample, conventional immunoassays are laborious and time consuming. The objective is to develop more efficient immunoassays for serosurveillance of nonhuman primates. Accordingly, we have developed a novel multiplex serodiagnostic system where individually identifiable, fluorescent microbeads are coated with purified whole viral antigens.