PHYSICAL MECHANISMS OF PULSED LASER MICROBEAM INTERACTIONS WITH CELLS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。使用时间分辨的Brightfield、相位对比和荧光显微镜方法，我们正在研究细胞裂解、细胞手术以及向单个细胞(光注射)或整个细胞群(光学定位)进行磨牙传递的物理机制。这些时间分辨技术允许在快至纳秒的时间尺度上可视化等离子体形成、冲击波传播、空化气泡形成和坍塌等过程。此外，外源荧光报告的使用使我们能够确定细胞对微束扰动的时间分辨响应。目前，对PtK2细胞系的研究主要集中在532 nm激光波长，脉冲宽度在200ps-5 ns的范围内。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Using time-resolved brightfield, phase contrast and fluorescence microscopy methods we are investigating the physical mechanisms responsible for cell lysis, cellular surgery, as well as molcular delivery to individual cells (optoinjection) or entire cell populations (optoporation). These time-resolved technqiues allow for visualization, on time scales as rapid as nanoseconds, processes such as plasma formation, shockwave propagation, cavitation bubble formation and collapse. Moreover, the use of exogenous fluorescence reporters enable us to determine the time-resolved cellular response to the microbeam perturbations. Currently, studies are focused on the PtK2 cell line using the 532 nm laser wavelength with pulse durations in the range of 200ps - 5ns.