AUTOMATIC CENTERING OF PROTEIN CRYSTALS USING X-RAYS

本子项目是利用由NIH/NCRR资助的中心赠款提供的资源的众多研究子项目之一。子项目和研究者（PI）可能已经从另一个NIH来源获得了主要资金，因此可以在其他CRISP条目中表示。列出的机构是中心的，不一定是研究者的机构。为了使晶体筛选过程完全自动化，开发和探索了蛋白质晶体与x射线束自动对齐的方法。样品可以使用平均I/Sig(I)对x射线束中不同晶体位置拍摄的图像进行自动对齐。使用图像分析程序DISTL对图像进行分析，以估计I/Sig(I)。人们发现，斑点的数量可以用来排列晶体。为优化对准工艺，对参数进行了细化。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In order to fully automate the crystal screening process, methods to automatically align protein crystals to the x-ray beam were developed and explored. Samples can be aligned automatically using average I/Sig(I) for images taken at various crystal positions in the x-ray beam. Images were analyzed using the image analysis program, DISTL, in order to estimate I/Sig(I). It was found that the number of spots could be used to align crystals. Parameters were refined for optimize the alignment process.