TRANSPORT GENE EXPRESSION AND DRUG ACCUMULATION IN MILK

该子项目是利用NIH/NCRR资助的中心赠款提供的资源的许多研究子项目之一。子项目和研究者（PI）可能从另一个NIH来源获得主要资金，因此可以在其他CRISP条目中表示。所列机构为中心机构，不一定为研究者机构。该方案的目标是系统地评估基因在药物积累到人乳中的作用。通过确定哪些转运蛋白存在于乳腺上皮细胞中，我们希望不仅能确定呋喃妥因和西咪替丁转运蛋白的身份，而且将未来的研究重点放在那些可能积累到乳汁中的药物类别上。功能研究将证实这些表达的转运蛋白在呋喃妥因和西咪替丁特异性蓄积中的作用。进一步开发模拟人乳腺上皮细胞屏障的模型细胞系统将显著增强我们对药物转运至乳汁中的机制的理解。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. The goal of the protocol is to systematically evaluate the role of genes in the accumulation of drugs into human milk. By identifying which transporters are present in mammary epithelial cells we hope to establish not only the identity of transporters for nitrofurantoin and cimetidine, but to focus future studies on those classes of drugs likely to accumulate into milk. Functional studies will substantiate the role of these expressed transporters in the specific accumulation of nitrofurantoin and cimetidine. The further development of a model cell system that mimics the human mammary epithelial cell barrier will significantly enhance our understanding of the mechanisms of drug transport into milk.