BLOOD-DERIVED DENDRITIC CELLS AND EPITHELIAL CELLS

这个子项目是利用由NIH/NCRR资助的中心拨款提供的资源的许多研究子项目之一。子项目和调查员(PI)可能从另一个NIH来源获得了主要资金，因此可能会出现在其他CRISE条目中。列出的机构是针对中心的，而不一定是针对调查员的机构。本研究探讨树突状细胞(DC)与呼吸道上皮细胞共培养的细胞生物学。目的是确定不同的DC亚型是否预测对周围颗粒物的免疫反应(Th1与Th2)。从血液和支气管镜中提取的原代细胞将进行体外培养。这项极具创新性的研究应该会提供重要的基本信息。研究人员将建立一个新的实验室系统，在这个系统中，同种异体a)血单核细胞或b)血CD34阳性脐带血细胞在两室装置中培养，允许这些细胞在不同细胞因子、Fit3配体、环境空气颗粒物等不明混合物的存在下接触分化的呼吸道上皮细胞。将通过未指明的方法监测血液单核细胞或CD34阳性细胞，以确定这些细胞的分化可能受到上皮细胞和细胞混合物暴露的刺激的各种可能方式的影响。上皮细胞对前体细胞分化为未成熟和成熟DC的影响是一个重要而值得研究的问题。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. This study investigates the cell biology of dendritic cells (DC) in co-culture with airway epithelial cells. The objective is to determine if different DC subtypes predict the immune response (Th1 vs. Th2) to ambient particulate matter. Primary cells derived from blood and bronchoscopy will be cultured ex vivo. This highly innovative study should provide important basic information. The investigators will set up a new laboratory system in which allogeneic a) blood monocytes or b) blood CD34-positive cord-blood cells are cultured in a two-chamber apparatus that permits these cells to contact differentiated respiratory epithelial cells in the presence of unspecified mixtures of different cytokines, FIT3 ligand, ambient air particulate matter, etc. The blood monocytes or CD34-positive cells will be monitored by unspecified means to determine the various possible ways that the differentiation of these cells might be influenced by the epithelial cells and the stimuli to which the cell mixtures are exposed. The issue of the influence of epithelial cells on the differentiation of precursor cells into immature and mature DC is important and worthy of study.