MOLECULAR DEFINITION OF FILARIAL AND RELATED NONFILARIAL GENES AND PROTEINS

该项目的目标是确定和产生丝虫病， 类圆线虫蛋白质在诱导寄生虫特异性 人类宿主的免疫反应，并在分子水平上 水平，相关蠕虫物种之间的差异。 重组 已经鉴定了抗原和探针， 和潜在的保护性分子。bancrofti; B）可以区分 在相关的丝虫物种中; c）识别W. bancrofti，O.扭转和Loa loa基因组; d）具有潜在的诊断价值 （e）负责直接的诱导; 丝虫感染中的超敏反应。此外，cDNA 文库的丝状和杆状幼虫的S。粪虫已经被 制作并表征，并构建了系统发育树， 这个寄生虫使用5S核糖体序列。

The objectives of this project are to define and generate filarial and strongyloides proteins that are important in inducing parasite-specific immune responses in the human host and to understand, at a molecular level, the differences among related helminth species. Recombinant antigens and probes have been identified that a) encode immunoreactive and potentially protective molecules of W. bancrofti; b) can distinguish among related filarial species; c) identify repeated segments of the W. bancrofti, O. volvulus and Loa loa genome; d) are of potential diagnostic importance; and e) are responsible for the induction of immediate hypersensitivity type responses in filarial infections. Further, cDNA libraries of filariform and rhabdiform larvae of S. stercoralis have been made and characterized and phylogenetic trees have been constructed for this parasite using 5S ribosomal sequences.