GENETIC MAPPING OF MOUSE CHROMOSOMAL GENES

我们集团致力于发展高密度的长期目标。 老鼠的基因图谱。我们一直在使用种间(亚)杂交。 为了生成一个几乎完全由表达的基因和 假基因。这些基因是通过对其后代的分析而定位的 两组遗传杂交--种间回交和 亚种间回交。这些小鼠的DNA已经被鉴定了好几次了 900个基因座，其中大约一半也被定位在其他 系统。这使得我们可以将新定义的基因映射到特定的 链接地图上的位置，并将我们的数据集成到合成中 每条染色体的图谱。这些研究已经导致了基因 今年绘制了数百个新基因的图谱，其中包括编码基因 钠和钙通道，锌指蛋白，淋巴毒素受体， 小鼠BRCA1，Tetranectin，巨噬细胞炎症蛋白，IL-2受体 亚单位和突触集合体。特定的地图位置可能很有用 因为接近已知的发育突变的信息可以 确定这样一个基因是异常表型的潜在候选基因。 其他研究主要集中在多基因家族的组织上。 哺乳动物基因组及其比较连锁关系的研究 人类和老鼠的同源基因。

Our group is involved in the long-term goal of developing a high density genetic map of the mouse. We have been using inter(sub)species crosses to generate a map consisting almost exclusively of expressed genes and pseudogenes. These genes are mapped by the analysis of the progeny of two sets of genetic crosses - an interspecies backcross and an intersubspecies backcross. DNAs from these mice have been typed for over 900 loci, about half of which have also been genetically mapped in other systems. This permits us to map newly defined genes to specific positions on the linkage map and to integrate our data into composite maps of each chromosome. These studies have resulted in the genetic mapping of several hundred new genes this year including genes encoding sodium and calcium channels, zinc finger proteins, lymphotoxin receptor, mouse BRCA1, tetranectin, macrophage inflammatory protein, IL-2 receptor subunits, and synaptotagmins. Specific map locations can be useful information since proximity to a known developmental mutation can identify such a gene as a potential candidate for the abnormal phenotype. Other studies have focused on the organization of multigene families in the mammalian genome and on the comparative linkage relationships of homologous genes in man and mouse.