ISOLATION AND SEROTYPIC CHARACTERIZATION OF HUMAN AND ANIMAL ROTAVIRUSES

在这个项目中，我们确定了各种抗原的主要抗原关系。 来源于人和动物的轮状病毒株。 阐明 轮状病毒的中和特异性很重要， 更全面地了解轮状病毒流行病学， 制定有效的疫苗接种策略。 通过使用 各种单基因替换人x人或人x牛 轮状病毒抑制剂和超免疫豚鼠抗血清 我们成功地鉴定了VP 4 代表性的人轮状病毒株的中和特异性（Wa， DS-1、ST 3、K8和69 M），并建立了五种不同的VP 4（P）血清型 基于应用于以下的>20倍抗体差异标准： VP 7血清型的分类。 两种兔VP 4衣壳蛋白的中和特异性 最初在美国分离的轮状病毒（C11和Ala）被发现 与人轮状病毒VP 4呈双向抗原相关 VP 7血清型6 PA 169菌株（分离自意大利）和VP 7血清型 8株HAL 1160（分离自芬兰）。

In this project we determined major antigenic relationships of various rotavirus strains derived from humans and animals. The elucidation of the neutralization specificities of rotavirus is important in order to achieve a more comprehensive understanding of rotavirus epidemiology and for formulation of an effective strategy for vaccination. By using various single gene substitution human x human or human x bovine rotavirus reassortants and hyperimmune guinea pig antiserum raised against each reassortant, we successfully characterized the VP4 neutralization specificity of representative human rotavirus strains (Wa, DS-1, ST3, K8, and 69M) and established five distinct VP4(P) serotypes based on a >20-fold antibody difference criterion that is applied to classification of VP7 serotypes. Neutralization specificities on the VP4 capsid protein of two rabbit rotaviruses (C11 and Ala) originally isolated in the U.S. were found to be related antigenically in a two-way fashion to human rotavirus VP4 of VP7 serotype 6 PA169 strain (isolated in Italy) and of the VP7 serotype 8 HAL1160 strain (isolated in Finland).