MARSHALL U COBRE: GENOMICS COREP=C

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 COBRE应用程序中的每个研究项目都严重依赖于基因表达 分析其具体目标的实现情况。最常见的基因组 本申请的方法是通过微阵列、单个mRNA 通过实时PCR和DNA测序和序列分析测量丰度。 微阵列实验和DNA测序需要昂贵的仪器， 试剂、复杂的方法以及密集的数据管理和分析。这些任务 由核心设施人员执行效率最高。拟议的COBRE项目 如果不能直接获得专家基因组技术和分析， 支持.因此，本申请的目的是实现基因组核心 满足COBRE和马歇尔大学具体需求的设施 基于微阵列的基因表达谱，实时PCR， 自动DNA测序和DNA序列分析。微阵列管理员 和基因组数据分析师将需要大量的外部培训，以跟上 并为最终用户提供有用的分析。的长期目标 基因组学核心设施是定位COBRE研究人员发现新的分子 癌症发展的途径。我们将使基因组研究的目标， COBRE研究项目通过追求以下两个具体目标：1。实施 基因组学核心设施和发展微阵列数据分析能力。的 基因组克隆设施将由奈尔斯博士和普里梅拉诺博士共同指导， 实验设计和核心管理方面的互补技能。考虑到你的经验 的共同董事和存在的关键人员和仪器，我们很好 准备开发基因组核心设施我们希望基因组学 核心设施将使COBRE和马歇尔大学的调查人员能够确定 候选基因在细胞信号转导中起关键作用，证实了 表达，并获得深入了解癌症分子生物学。doF？

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Each research project within this COBRE application relies heavily on gene expression analysis for the completion of their specific aims. The most common genomic approaches in this application are gene expression profiling by microarray, single mRNA measurement abundance by real-time PCR, and DNA sequencing and sequence analysis. Microarray experimentation and DNA sequencing require expensive instrumentation and reagents, sophisticated methods, and intense data management and analysis. These tasks are most efficiently executed by core facility personnel. The proposed COBRE projects cannot be completed without direct access to expert genomic technical and analytical support. Therefore, the objective of this application is to implement a Genomic Core Facility that will serve the specific needs of the COBRE and Marshall University investigations in the areas of microarray-based gene expression profiling, real-time PCR, automated DNA sequencing and DNA sequence analysis. Both the microarray manager and genomic data analyst will require substantial external training to keep pace with developments and provide useful analyses for the end users. The long-term goal of the Genomics Core Facility is to position COBRE investigators to discover novel molecular pathways in cancer development. We will enable the genomic research goals of the COBRE research projects by pursuing the following two specific aims: 1. Implement a Genomics Core Facility and develop Microarray Data Analysis capabilities. The Genomic C o re Facility will be co-directed by Drs Niles and Primerano who have complementary skills in experimental design and core management. Given the experience of the co-directors and presence of key personnel and instrumentation, we are well prepared to develop the Genomics Core Facility. It is our expectation that the Genomics Core Facility will enable COBRE and Marshall University investigators to identify candidate genes that are critically involved in cell signal transduction, confirm pattems of expression, and gain insights into cancer molecular biology.t?doF¿