RECOVERY OF EPIDIDYMAL SPERM FROM RHESUS MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景资料：为了确定是否可以回收附睾精子并成功冷冻保存，在尸检时从总共20只恒河猴中收集睾丸。方法：男性，年龄3 ~ 19岁，平均8.5岁。 未从3只小于4岁的动物中获得精液。其余17份样本所含精液的平均精子细胞浓度为144. 0 mio/mL，平均活力为72. 8%，平均等级为2. 6。结果/讨论：培养24小时后，无论处理如何，所有样本的总体活力均显着下降，24小时时新鲜非活化精液为8.8%，活化精液为3.8%。冷冻对精液有显着影响，平均等级降低至1.6，解冻后即刻活力降低至42.4%。 激活进一步降低新鲜精液4小时后的精子活力（72.8比50.4%），但增加它在精液冷冻（14.0比23.2%）。男性的年龄并不影响精液浓度，等级或初始活力，但被证明是一个重要的因素，在确定冻融激活精液的活力较低的活力，发现从老年男性的样本。总的来说，这项研究表明，可以从死后的男性获得活动的精液，但随后的研究将不得不确定是否质量足以促进其在辅助生殖中的使用。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Background: To determine whether epididymal sperm could be retrieved and successfully cryopreserved testes were collected at necropsy from a total of 20 rhesus macaques. Methods: The males ranged in age from 3 to 19 years with an average of 8.5 years. No semen was obtained from three animals, which were younger than 4 years. The remaining 17 samples contained semen with an average sperm cell concentration of 144.0 mio/mL, an average motility of 72.8 % and a mean grade of 2.6. Results/Discussion: After 24 h of culture the overall motility decreased significantly in all samples regardless of treatment to 8.8 % in fresh non-activated and 3.8 % in activated semen at 24 hr. Freezing had a significant effect on semen reducing the mean grade to 1.6 and the immediate post-thaw motility to 42.4 % in non-activated samples. Activation further reduced semen motility after 4 hr in fresh semen (72.8 vs 50.4%), but increased it in semen that had been frozen (14.0 vs 23.2%). The age of the male did not influence semen concentration, grade or initial motility, but proved to be a significant factor in determining motility of frozen-thawed activated semen with lower motility found in samples from older males. Overall the study demonstrates that motile semen can be obtained from post-mortem males, although subsequent studies will have to determine whether the quality is sufficient to facilitate its use in assisted reproduction.