LASER MICROBEAM-INDUCED SPATIOTEMPORAL CHANGE IN REFRACTIVE INDEX

这个子项目是许多研究子项目中利用 资源由NIH/NCRR资助的中心拨款提供。子项目和 调查员(PI)可能从NIH的另一个来源获得了主要资金， 并因此可以在其他清晰的条目中表示。列出的机构是 该中心不一定是调查人员的机构。 我将测量能量和辐射阈值，以诱导有丝分裂的PTK2(三指)细胞染色体微照射区域的折射率变化，以期深入了解激光消融的物理机制。我将确定不同数量的脉冲在不同波长的损坏斑点的宽度。此外，对于固定的波长，我将测量在不同曝光时间下引起折射率可检测变化所需的时间，以确定任何时间相关性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. I will measure energy and irradiance thresholds for inducing a change in the refractive index of micro-irradiated regions of chromosomes in mitotic PTK2 (Potorous tridactylus) kidney cells for the purpose of gaining insights into the physical mechanisms of laser ablation. I will determine the width of damaged spots for different numbers of pulses at different wavelengths. Further, for a fixed wavelength, I will measure the time required to induce a detectable change in the refractive index with varying exposure times to determine any time dependence.