High Resolution Non-Invasive Imaging of Oral and Craniofacial Tissues

描述(申请人提供)：本申请是为了获得一台微型X射线计算机断层摄影仪(MicroXCT-200)。计算机断层扫描的最新进展为生物材料和生物工程研究打开了新的机遇。微CT的发展使得在原位条件下(模拟体内)高分辨率成像矿化组织的内部结构及其与软组织的界面，分辨率可达1&lt；m。这些能力是在分析生物组织和生物材料以了解其固有特征方面的突破，这些特征构成了用于修复、植入和组织再生的新一代智能材料开发的组成部分。MicroXCT-200是在三个空间维度进行定量微结构和矿物含量评估的最通用仪器之一。这一多用户设施将极大地加强七名NIH研究人员以及其他几名全职研究人员的研究。目前校园中可用的微CT设备分辨率有限，使用率为100%，能力更差。请求提供资金，用于购买最先进的MicroXCT-200，XRadia Inc.系统。这项提议得到了加州大学旧金山分校、旧金山国立卫生研究院、UOP、LBNL和SLAC教职员工的广泛支持；来自代表8个PI的实验室小组的人员，目前由NIH提供资金，作为计划用户。拟议的MicroXCT-200将被安置在美国国防部生物材料和生物工程系的纳米组织表征核心设施中。加州大学旧金山分校牙科学院口腔预防与修复科学系。B&B事业部的核心设施利用高分辨率原子力和扫描电子显微镜技术支持生命科学、材料科学和化学科学的跨学科研究，将保证仪器处于最佳状态的维护和操作，并将为赠款提案中指定的主要用户、次要用户和未来用户提供出色的培训和支持。收购这一仪器将推动在现有赠款方面取得实质性进展，这些赠款包括了解健康和疾病中的牙周复合体、骨骼疾病、龋齿、再矿化方法、使用仿生学和组织工程原理的组织再生、干细胞方法，以及为将要研究的项目开发用于临床牙科的非电离成像。 公共卫生相关性：这笔赠款要求新的高倍率非侵入性成像设备，以支持NIH支持的一些研究人员的关键工作，这些研究人员在牙齿和骨骼疾病领域工作，并开发可将所需手术降至最低的治疗方法，牙齿腐烂的新诊断程序，以及组织工程和干细胞，以帮助牙齿、口腔和骨骼问题的再生。

DESCRIPTION (provided by applicant): This application is for acquisition of a micro X-ray computed tomograph (MicroXCT-200). Recent advances in computed tomography open new opportunities for biomaterials and bioengineering research. The developments of microCTs allow high resolution imaging of internal structures of mineralized tissues and their interfaces with soft tissues down to 1 <m under in situ conditions (simulated in vivo). These capabilities are a breakthrough in the analysis of biological tissues and biomaterials to understand their native characteristics which constitute an integral part in the development of new generation of smart materials for restoration, implantation and tissue regeneration. The MicroXCT-200 is one of the most versatile instruments for a quantitative microstructural and mineral content evaluation in three spatial dimensions. This multiuser facility will greatly enhance the research of seven NIH investigators, as well as several other full-time researchers. The microCTs facilities currently available on campus are limited in resolution with 100% usage and much less capable. Funding is requested for the acquisition of a state-of-the-art MicroXCT-200, Xradia Inc., system. The proposal has received broad based support from UCSF, SFGH, UOP, LBNL and SLAC faculty; personnel from laboratory groups representing 8 PI's with current funding from NIH as projected users. The proposed MicroXCT-200 will be housed in the Nanoscale Tissue Characterization Core Facility in the Division of Biomaterials and Bioengineering, Dept. of Preventive and Restorative Dental Sciences, School of Dentistry, UCSF. The core facility in the Division of B&B has championed interdisciplinary research with the programs in the life sciences, materials sciences, and chemical sciences using high resolution atomic force and scanning electron microscopy techniques and will guarantee maintenance and operation of the instrument in top condition, and also will provide excellent training and support for the major users named on the grant proposal, and minor and future users. Acquisition of this instrument will promote substantial progress on current grants that include understanding of the periodontal complex in health and disease, bone disease, dental caries, and methods of remineralization, tissue regeneration using biomimetics and tissue engineering principles, and stem cell approaches, as well as the development of non-ionizing imaging for clinical dentistry for the projects to be studied. PUBLIC HEALTH RELEVANCE: This grant requests new high magnification non-invasive imaging equipment to support critical work of a number of NIH supported investigators who are working in the areas of dental and bone disease and the development of treatments that will minimize needed surgery, new diagnostic procedures for dental decay, and tissue engineering and stem cells to aid in regeneration of dental, oral, and skeletal issues.