Request for Zeiss 710 multiphoton 5Live Duoscan Microscope

描述（由申请人提供）：此请求是为购买多光子/单光子高速共聚焦显微镜的资金。该仪器将存放在匹兹堡大学医学院的生物成像中心（CBI）内。该核心设施的任务是为匹兹堡大学医学院的所有研究小组提供全方位的光和电子光学，图像分析和形态测量方法。 光学切片显微镜是该中心提供的一项基本服务，该中心目前使用的核心共聚焦显微镜是一台3年和2年的Olympus FV 1000，一台9年的Olympus fluoview 500，一台同样老化的Leica TCS SL和一台6年的Zeiss 510。我们最近还获得了倒置单光子/多光子Olympus FV 1000。该应用的原因是：1：为无法使用CBI内当前倒置系统进行研究的系统提供使用直立配置的多光子显微镜的访问2：为各种项目提供高速共焦成像，从在开发模型系统（如鱼和小鼠）中成像血流，人气道培养物中的毛细血管搏动和脑切片中的钙测量自从CBI 17年前开始运作以来，它已经成为医学研究界的一个组成部分，目前参与医学领域内200多个PHS资助团体的研究项目，以及在公共卫生系统支持的项目与其他部门的调查人员在邻近机构，并与合作者在全国各地。这里要求的设备是一个重要的和基本的除了显微镜的军械库提供给CBI的用户，它将有基本的和重大的影响，在匹兹堡大学和超越PHS资助的研究。

DESCRIPTION (provided by applicant): This request is for funds to purchase a multiphoton capable/single photon high speed confocal microscope. This instrument will be housed within the Center for Biologic Imaging (CBI) at the University of Pittsburgh School of Medicine. The mandate of this core facility is to provide access to a full range of light and electron optical, image analysis, and morphometric methods to all research groups within the University of Pittsburgh School of Medicine. Optical sectioning microscopy is an essential service provided by the center, the current core use confocal microscopes within the facility are a 3 yr old and a 2 yr old Olympus FV1000, a 9 year old Olympus fluoview 500, a similarly aged Leica TCS SL and a 6 year old Zeiss 510. We have also recently aquired an inverted single photon/multiphoton Olympus FV1000. The reasons for this application are to: 1: Provide access to multiphoton microscopy using an upright configuration for systems which cannot be studied using the inverted system currently within the CBI 2: Provide access to high speed confocal imaging for a variety projects ranging from imaging blood flow in developing model systems such as fish and mice, cillary beat in human airway cultures and calcium measurements in brain slices Since the CBI commenced operations 17 years ago, it has become an integral part of the medical research community currently participating in research projects with more than 200 PHS funded groups within the medical area, as well as in PHS supported projects with investigators in other departments at neighboring institutions, and with collaborators around the country. The device requested here is an important and fundamental addition to the armamentarium of microscopies available to users of the CBI and it will have fundamental and significant impact on PHS funded research at the University of Pittsburgh and beyond.