COBRE: LOUISVILLE RES FOUND INC: CORE B: MICROARRAY FACILITY

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 微阵列设施位于Baxter II生物医学研究大楼，距离布朗癌症中心一个街区。该设施包含Affymetrix和Agilent微阵列仪器系统，可供所有MT-Cobre成员使用。该机制由沃尔夫冈·扎卡里亚斯博士领导，自2001年春以来已在过去八年中全面运作。它包括一名全职设施经理Sabine Waigel，以及两名研究技术人员Vennila Arumugam和李晓红。它们提供行政支助、项目后勤和管理、特定平台样品处理和杂交方面的技术援助，以及数据收集、分析和储存。Zacharias博士可以在实验设计、数据分析以及资助申请的协作或顾问支持方面提供帮助。 微阵列设施与其他核心设施和生物信息学人员有密切的互动。此外，与J.G.布朗癌症中心的组织生物库的密切关系使其能够制备具有已知病理特征的样本和适当的对照样本。开发该设施的组织阵列组件的计划也在进行中。高分辨率Affymetrix扫描仪3000升级允许所有Cobre研究人员使用最新一代高密度全基因组v2.0阵列和SNP 6.0阵列，用于人类或小鼠。该机制继续在校园内组织和举办软件/数据分析讲习班，以培训微阵列技术用户。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. The Microarray Facility is located in the Baxter II Biomedical Research Building, one block from the Brown Cancer Center. This facility contains both Affymetrix and Agilent microarray instrument systems which are available to all MT-COBRE members. The Facility is under the direction of Dr. Wolfgang Zacharias and has been fully operational for the past eight years since Spring 2001. It includes a full-time Facility Manager, Sabine Waigel, and two research technologists, Vennila Arumugam and Xiaohong Li. They provide administrative support, project logistics and management, technical assistance in platform-specific sample processing and hybridization, as well as data collection, analysis and storage. Dr. Zacharias is available for assistance with experimental design, data analysis, and collaborative or consultant support for grant applications. The Microarray Facility has close interactions with other Core Facilities and Bioinformatics personnel. Also, the close relationship with the Tissue Biorepository in the J.G. Brown Cancer Center allows the preparation of samples with known pathologic characteristics and appropriate control specimens. Plans are underway for the development of a tissue array component of this facility as well. The high-resolution Affymetrix Scanner 3000 upgrade allows all COBRE investigators to use the newest generation of high-density whole genome v2.0 arrays and SNP 6.0 arrays for human or mouse. The Facility continues to organize and hold software/data analysis workshops on campus to train microarray technology users.