REDOX IMAGING OF HUMAN LYMPHOMA

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 氧化还原扫描可以根据NADH和FP(氧化黄素蛋白)的荧光信号来测量体内线粒体的氧化还原状态，FP氧化还原比率(FP/(NADH+FP))是预测人类黑色素瘤和乳腺癌在小鼠移植瘤中侵袭性的指标。氧化还原比对细胞过程也很敏感，包括细胞凋亡、分化和新陈代谢。在这个项目中，我们使用氧化还原扫描来研究人类淋巴瘤细胞培养和有无化疗的小鼠异种移植。我们希望更多地了解线粒体氧化还原状态在肿瘤进展和治疗反应中的作用。我们还可能为以线粒体氧化还原状态为靶点作为一种新的癌症治疗方法积累证据。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. Redox scanning can measure the in vivo mitochondrial redox states of tissues on the basis of the fluorescence signals of NADH and Fp (oxidized flavoproteins), and Fp redox ratios (Fp/(NADH+Fp)) was shown by previous studies to predict the aggressiveness of human melanoma and breast cancer in mouse xenografts. Redox ratio is also sensitive to cellular processes including apoptosis, differentiation and metabolism. In this project we employ redox scanning to study human lymphoma cell culture and mouse xenografts with and without chemotherapy. We hope to understand more about the role of mitochondrial redox state in tumor progression and response to treatment. We may also accumulate evidence for targeting mitochondrial redox state as a novel approach of cancer therapy.