TRACHEAL CARTILAGE RESHAPING

这个子项目是利用资源的许多研究子项目之一。 由NIH/NCRR资助的中心拨款提供。对子项目的主要支持 子项目的首席调查员可能是由其他来源提供的， 包括美国国立卫生研究院的其他来源。为子项目列出的总成本可能 表示该子项目使用的中心基础设施的估计数量， 不是由NCRR赠款提供给次级项目或次级项目工作人员的直接资金。 前言：常见的气管前壁塌陷的外科修复技术(所谓的“开放技术”)往往伴随着较高的发病率，而长时间的支架置入术会加重持续的气管炎症，并成为新的狭窄的潜在来源。目前，我们正在研究内窥镜下激光软骨成形术的激光剂量测量和手术装置的设计，以最大限度地减少软骨组织的热损伤。目的：为了更好地了解激光诱导的气管软骨重塑，我们研究了组织活性与激光剂量学之间的关系以及内窥镜下激光照射引起的力学性质的变化。此外，我们还设计了一种用于激光气管软骨重塑的有效的内窥镜手术装置。材料和方法：取新西兰大白兔气管软骨，用Candela公司生产的半导体激光(波长1450 nm，0.03~0.14W/mm2)照射3~5s。使用ELF 3200机械测试仪(EnduaTEC，Minnetonka，MN)测量机械性能的变化，并使用激光扫描显微镜(LSM510Meta，Carl Zeiss Inc.，德国)确定其活性。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. Primary support for the subproject and the subproject's principal investigator may have been provided by other sources, including other NIH sources. The Total Cost listed for the subproject likely represents the estimated amount of Center infrastructure utilized by the subproject, not direct funding provided by the NCRR grant to the subproject or subproject staff. INTRODUCTION:The usual surgical repair techniques (so-called "open technique") for anterior tracheal wall collapse are often associated with a high rate of morbidity, and prolonged stenting can aggravate the ongoing tracheal inflammation and serve as a potential source for a new stenosis. We are currently studying the laser dosimetry and the design of the surgical device for endoscopic laser cartilage reshaping minimizing the thermal damage of cartilage tissue. OBJECTIVE: In order to provide a better understanding of laser-induced tracheal cartilage reshaping, we examined the relationships between the tissue viability and the laser dosimetry as well as the change in mechanical property due to endoscopic laser exposure. In addition, we design an effective endoscopic surgical device for laser tracheal cartilage reshaping. MATERIALS AND METHODS: Tracheal cartilage extracted from New Zealand rabbits, were exposed to diode laser (wavelength =1450nm, 0.03-0.14W/mm2, Candela Corp. MA) for 3-5 seconds. The change in the mechanical property was measured using an ELF 3200 mechanical testing instrument (EnduraTEC, Minnetonka, MN) and its viability was determined using a laser scanning microscope (LSM510 Meta, Carl Zeiss Inc., Germany).