WaNPRC Macaca nemestrina SPF Breeding Colony

描述(由申请人提供)：该项目的目标是维持和扩大无特定病原体(SPF)的小尾猴(Macaca Nomestrina)繁殖群体，以支持华盛顿国家灵长类动物研究中心(WaNPRC)由NlH支持的研究。猕猴是人类疾病的宝贵模型，也是生物医学研究的重要资源。奈米斯特氏菌具有独特的特性，这使它们具有无价的价值，特别是在艾滋病发病机制、免疫学、疫苗开发和阴道杀菌剂评估方面。WaNPRC拥有国内唯一一个主要的nememstrina繁殖地。近年来，为了满足WaNPRC对新月藻的需求，有必要从印度尼西亚进口动物。即使有了进口，满足NLH支持的研究对动物的要求也是一个挑战。我们建议在未来四年内，将我们的国内SPF繁殖群体扩大到1400只动物的自给自足的普查，这将满足对新月形吸虫的预期需求。我们将采取两个主要战略来满足这一需求：改善管理和进口繁殖动物。新月形猕猴不像其他猕猴那样耐寒，有不同的繁殖要求。我们最近在亚利桑那州建立了一个由WaNPRC直接管理的nememstrina繁育设施，并专门专注于该物种。我们预计，通过这一战略，我们将能够降低死亡率和提高生育率，从而导致该群体中动物数量的净增加。我们还将进口200只新月藻，以达到我们扩大种群的目标。我们将在运输前对进口动物进行病毒抗体的血清学检测，并通过内部重复的血清学测试来确认它们的无病原体状态。干扰研究结果的病毒病原体(SIV、STLV、SRV)或对人类构成人畜共患风险的病毒病原体(McHV-1，也称为B型疱疹病毒)将从群体中消除，方法是将未感染的后代与受感染的后代分开饲养，并定期对群体中的所有动物进行检测。

DESCRIPTION (provided by applicant): The goal of the proposed project is to maintain and expand a specific-pathogen free (SPF) breeding colony of pigtail macaques (Macaca nemestrina) for the support of NlH-supported research at the Washington National Primate Research Center (WaNPRC). Macaques are a valuable model of human disease and are an essential resource for biomedical research. M. nemestrina possess unique characteristics that make them invaluable, particularly in the study of AIDS pathogenesis, immunology, vaccine development, and vaginal microbicide evaluation. The WaNPRC maintains the only major domestic breeding colony of M. nemestrina. In order to meet the demand for M. nemestrina at the WaNPRC in recent years, it has been necessary to import animals from Indonesia. Even with the importation, it has been a challenge to meet requests for animals for NlH-supported research. We propose over the course of the next four years to expand our domestic SPF breeding colony to a self-sustaining census of 1400 animals which will meet the anticipated demand for M. nemestrina. We will employ two major strategies to meet this demand: Improved management, and importation of breeding animals. M. nemestrina are not as hardy as other macaque species, and have different breeding requirements. We have recently established a breeding facility for M. nemestrina in Arizona that is directly managed by the WaNPRC, and focuses specifically on that species. We anticipate that with this strategy we will be able to reduce mortality and increase fertility, resulting in a net increase in the number of animals in th colony. We will also import a total of 200 M. nemestrina to reach our goal of colony expansion. We will have imported animals tested serologically for viral antibodies prior to shipment and confirm their pathogen-free status with in-house repeated serologic testing. Viral pathogens that interfere with research results (SIV, STLV, SRV) or are a zoonotic risk to humans (McHV-1, also known as herpes B) will be eliminated from the colony by raising uninfected offspring separately from infected progenitors and testing all animals in the colony regularly.