Imaging Core

成像核心（core D）由一个集中的尖端显微镜设备组成，对于这个P01应用程序中的项目的研究目标至关重要，并位于匹兹堡大学生物成像中心（CBI） （www.cbi.pitt.edu）内。所有调查人员都已经并将继续大量使用CBI作为特定设施的成像方法。这种长期使用的证据可以在PI与核心工作人员（沃特金斯，圣克罗伊，斯托尔兹）以及单个项目的PI共同撰写的出版物中看到。CBI提供的成像专业包括所有超微结构电子显微镜（透射电子显微镜，扫描电子显微镜，免疫电子SEM和TEM），光和荧光显微镜（宏观解剖光和荧光，外因荧光，共聚焦扫描和多光子成像），活细胞显微镜（透射光和荧光）和荧光专业，如FRET， FRAP光谱分析和比例成像。同样关键的数据处理，广泛的图像分析软件和技术援助是可用的程序调查员。

The Imaging core (Core D) is comprised of a centralized cutting-edge microscopy facility, absolutely essential to the research goals of Projects in this P01 application and housed within the Center for Biologic Imaging (CBI) (www.cbi.pitt.edu) of the University of Pittsburgh. All investigators have made, and continue to make, heavy use of the CBI for facility-specific imaging methodologies. Evidence of the longevity of this use is seen in co-authored publications between the PI and staff of the Core (Watkins, St. Croix, Stolz) and PI's of the individual projects. The imaging specialties afforded by CBI include all ultrastructural electron microscopy (transmission electron microscopy, scanning¿ electron microscopy, immune-electron SEM and TEM), light and fluorescence microscopy (macro dissecting light and fluorescence, epi-fluorescence, confocal scanning and multi-photon imaging), live cell microscopy (transmitted light and fluorescence) and fluorescence specialties like FRET, FRAP spectral analysis and ratiometric imaging. Also critical to data processing, a wide range of image analysis software and technical assistance is available to program investigators.