A Novel Multilayered Membrane for Periodontal Regeneration

描述(申请人提供)：随着美国人口老龄化，重要的是寻找新的牙科生物材料，以促进牙周再生，并将其转化为临床实践。作为一名牙医和材料科学家，申请者有能力弥合生物材料开发之间的差距。以及利用纳米技术、组织工程学和临床知识在病人护理中的应用。该奖项的目的是为首席研究员提供细胞和分子生物学、骨骼生物学和翻译研究方面的广泛培训，重点是牙周组织再生。这项申请中的实验旨在纳入新的方法，并将由一个多学科的专家团队指导，他们将提供必要的培训，以提高博蒂诺博士的科学技术和分析技能。首席研究员的最终目标是成为一名多产和独立的临床科学家，他可以获得联邦资金来赞助一个独立的研究项目。这项研究的中心假设是，通过添加磷酸钙和成纤维细胞生长因子-2(CaP/FGF2)和成纤维细胞生长因子2，能够以特定位置刺激各种组织类型的再生的多层膜将导致适当位置的牙槽和牙周组织再生。文献表明，任何单一的可植入生物材料都不能持续地引导多种组织类型的协调生长和发育，特别是在非常大的牙周缺损处。因此，迫切需要开发再生疗法。 促进口腔和颅面组织的再生和重建。这项研究的具体目的是(1)使用体外实验模型来最大化多层种植体表面层的组织特异性再生能力，(2)使用在体犬模型来评估CAP/FGF2对牙槽骨缺损中骨再生的有效性，以及(3)使用在体犬模型来评估多层种植体以特定位置的方式再生软组织和矿化组织的有效性。首席研究人员制定了一项全面的职业发展计划，其中包括正规课程、实验室指导，以涵盖密集的细胞和分子生物学培训，以及体内技术，以及在他的指导团队的监督下进行的与骨/牙周组织再生相关的各种分析。总体而言，K08奖项有能力提供所需的资源和培训，为申请者提供基础以及关键的理论和实践手段，使其成为一名能够将其生物材料知识转化为临床实践的高素质临床医生和科学家。

DESCRIPTION (provided by applicant): With the US population aging, it is important to find novel dental biomaterials that will promote periodontal regeneration, and translate these to clinical practice. As both a dentist and materials scientist, the applicant is in a position to brige the gap between biomaterials development. and their application in patient care using nanotechnology, tissue engineering and clinical knowledge. The purpose of this award is to provide the principal investigator extensive training in cellular and molecular biology, skeletal biology, and translational research with an emphasis on periodontal tissue regeneration. The experiments in this application have been designed to incorporate new methodologies, and will be guided by a multidisciplinary team of experts who will provide the required training to advance Dr. Bottino's scientific technical and analytic skills. The principal investigator's ultimae goal is to become a productive and independent clinician-scientist who can secure federal funding to sponsor an independent research program. The central hypothesis of the proposed research is that a multilayer membrane capable of stimulating the regeneration of various tissue types in a location specific manner, through the addition of calcium phosphate and fibroblast growth factor-2 (CaP/FGF2) and FGF2, will lead to alveolar and periodontal tissue regeneration in the appropriate locations. The literature indicates that no single implantable biomaterial can consistently guide the coordinated growth and development of multiple tissue types, especially in very large periodontal defects. Thus, there is a critical need to develop regenerative therapies to promote oral and craniofacial tissue regeneration and reconstruction. The specific aims of the proposed research are (1) To use an in vitro assay model to maximize the tissue-specific regenerative capacity of the multilayered implant surface layers, (2) To assess the effectiveness of CaP/FGF2 on bone regeneration in an alveolar defect using an in vivo canine model, and (3) To assess the effectiveness of the multilayered implant to regenerate soft and mineralized tissues in a location-specific manner using an in vivo canine model. The principal investigator has devised a comprehensive career development plan that includes formal coursework, laboratory guidance to cover intensive cell and molecular biology training, as well as in vivo techniques combined with a diverse set of bone/periodontal tissue regeneration related analyses under the supervision of his mentoring team. Collectively, the K08 award has the capacity to provide the resources and training needed to give the applicant the foundation and critical theoretical and practical means to become a highly qualified clinician-scientist who can translate his knowledge of biomaterials to clinical practice.