MAINTENANCE OF A CLOSED CPRC SPF COLONY

项目总结/摘要： 这项申请的主要目标是维持加勒比地区的无特定病原体（SPF）计划 灵长类动物研究中心（CPRC）通过支持运营，管理，兽医护理，病毒学 和MHC分型，并通过改进我们的综合遗传管理计划。的独特特征 CPRC动物有利于成功建立和可持续的SPF群体，这些动物是 天然不含SIV和SRV-D，这是四种待检测病毒中的两种。然而，为了安全和需要， 对于SPF计划，我们继续系统地检测所有四种病毒（B病毒、STLV、SIV和SRV-D）。的 计划提供具有明确遗传和病毒学背景的印度恒河猴。 群体遗传分析显示，SPF群体随着时间的推移保持遗传同质性 具有足够量的杂合性和来自其非SPF建立者的最小分层。遗传 使用本说明书中描述的遗传管理策略， 应用程序.遗传管理方法将允许富集所需的MHC标记物 生物医学研究，同时保持遗传多样性。我们的基因管理方法将是 与NIH的国家NHP遗传学和基因组学联盟指南一致，以确保长期 CPRC SPF菌落的长期存活率。SSFS SPF群体动物印第安血统的估计 也是几个国家灵长类动物研究中心（NPRCs）计算的最高值之一 在美国.本申请要求提供资金，以维持社会保障基金方案并实现拟议目标。 在NIH的持续支持下，机构捐款和来自计划收入的资金，SPF CPRC的一项计划将仍然是一个重要的国家研究资源，并将能够提供健康的SPF NIH赞助的研究中确定遗传和病毒学背景的印度恒河猴 程序.

PROJECT SUMMARY/ABSTRACT: The major goal of this application is to sustain the Specific Pathogen Free (SPF) program at the Caribbean Primate Research Center's (CPRC) through support of operations, administration, veterinary care, virological and MHC typing and by the improvement of our comprehensive genetic management plan. A unique feature of CPRC animals in favoring a successful establishment and sustainable of SPF colony are that these animals are naturally free of SIV and SRV-D, two of the four viruses to be tested for. Nevertheless, for safety and as required for the SPF Program, we continue systematically testing for all four viruses (B virus, STLV, SIV and SRV-D). The Program provides unadmixed Indian-origin rhesus monkeys with defined genetic and virological background. The population genetic analyses revealed that the SPF colony has remained genetically homogenous over time with sufficient amounts of heterozygosity and minimal stratification from its non-SPF founders. Genetic variation will be maintained in the SPF colony using the genetic management strategies described in this application. The genetic management approach will allow for enrichment of desired MHC markers in demand for biomedical research while maintaining genetic diversity. Our approach for genetic management will be concordant with the NIH's National NHP Genetics and Genomics Consortium guidelines for ensuring the long- term viability of the CPRC's SPF colony. The estimates of Indian ancestry among the SSFS SPF colony animals is also among the highest that have been calculated across several National Primate Research Centers (NPRCs) in the US. Funds are requested in this application to sustain the SPF program and to meet the proposed aims. With continued NIH support, the Institutional contribution and with funds from the program income, the SPF program at CPRC will remain a significant national research resource and will be able to provide healthy SPF indian-origin rhesus monkeys of defined genetic and virological background to NIH-sponsored research programs.