Core B: Non-Human Primate Core

NHP核心B摘要 非人灵长类(NHP)核心寻求巩固对NHP实验的监督和绩效 题为“针对RhCMV/SIV的保护性免疫机制规划”方案项目的议定书 疫苗和IL-15的作用“成为一个由经验丰富的研究人员组成的结构化核心。这个 核心的全球目标是提供领导和技术专长，以确保一致性和质量 动物选择的控制、研究方案的执行、实验程序的应用、动物 进行必要的观察和数据收集，以满足项目和其他核心调查人员的目标。 为此，核心将管理和直接监督项目的所有国家惠普研究，包括：1) 动物选择；2)动物住房和一般畜牧业；3)实验程序和临床 管理；4)标本收集和处理；5)尸检研究；6)采集和管理 动物人口统计学、生理学、临床和病理学数据。计划项目的总体目标 是确定系统对RhCMV/SIV疫苗接种的反应以及IL-15信号在编程中的作用 疫苗诱导的免疫反应导致SIV感染完全停止和随后清除 在RhCMV/SIV免疫的恒河猴中。NHP核心将提供专业知识和技术支持 需要确保成功完成六项多层面和广泛的国家卫生方案实验方案 支持本方案提出的2个项目。这些研究旨在：a)确定系统性 人巨细胞病毒/SIV疫苗诱导全血保护的转录相关研究--预测转录 外周和粘膜淋巴组织中的Signature[wbPPTS]及其对IL-15信号的依赖(项目 1)；b)确定对组织中感染RhCMV/SIV的细胞的特定空间转录反应，并确定 载体感染细胞对与wbPPTS相关的局部和系统转录反应的直接贡献 方案编制(项目1)；c)确定IL-15信号之间联系的潜在机制 WbPPTS的静止和生成(项目1)和d)连接组织和血液转录 确定的编程是a-c到IL-15信号通路、RhCMV/SIV疫苗的效力和较大的疫苗 国家卫生计划和人的应对情况(项目2)。

NHP CORE B SUMMARY The Nonhuman Primate (NHP) Core seeks to consolidate supervision and performance of the NHP experimental protocols of the Program Project entitled “Mechanisms Programming Protective Immunity from RhCMV/SIV Vaccine and IL-15 Actions” into a structured Core composed of highly experienced research personnel. The global objective of the Core is to provide leadership and technical expertise to ensure consistency and quality control in animal selection, execution of study protocols, application of experimental procedures, animal observations and data collection necessary to meet the objectives of Project and other Core lead investigators. To accomplish this, the Core will manage and directly supervise all NHP studies for the Project including: 1) animal selection; 2) animal housing and general husbandry; 3) experimental procedures and clinical management; 4) specimen collection and processing; 5) necropsy studies; and 6) acquisition and management of animal demographic, physiologic, clinical, and pathologic data. The overall objective of the Program Project is to determine the systems response to RhCMV/SIV vaccination and the role of IL-15 signaling in programming vaccine-induced immune responses responsible for complete arrest and subsequent clearance of SIV infection in RhCMV/SIV-immunized rhesus macaques. The NHP Core will provide the expertise and technical support required to ensure successful completion of the six multifaceted and extensive NHP experimental protocols supporting the 2 projects proposed in this Program. These studies are designed to: a) determine systemic transcriptomic correlates of the RhCMV/SIV vaccine-induced whole blood Protection-Predictive Transcriptomic Signature [wbPPTS] in peripheral and mucosal lymphoid tissues and its dependence on IL-15 signaling (Project 1); b) define specific spatial transcriptomic response to RhCMV/SIV-infected cells in tissues and identify the direct contribution of vector-infected cells to the local and systemic transcriptomic responses linked to wbPPTS programming (Project 1); c) determine the mechanisms underlying the association between IL-15 signaling quiescence and generation of the wbPPTS (Project 1) and d) link the tissue and blood transcriptomic programming identified is a-c to IL-15 signaling pathways, RhCMV/SIV vaccine efficacy and the larger vaccine response landscape in NHP and people (Project 2).