BREAST CARCINOMA GLYCOPROTEIN CHANGES CAUSED BY ONCOGENE EXPRESSION

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 人类癌基因her-2的表达导致N-乙酰葡糖胺转移酶T-V（GlcNAc T-V）的选择性增加 mRNA和活性。使用与GlcNAc T-V产物结合的植物凝集素对乳腺组织进行的组织学研究显示， 该产物不存在于非患病乳腺组织中，但在细胞出现肿瘤时表达。GlcNAc T-V 产物在肿瘤进展期间表达增加。该项目的重点是确定特定的糖蛋白， 表达GlcNAc T-V产物的乳腺活检组织，以确定乳腺癌的可能生物化学肿瘤标志物。 carcinoma.为了鉴定这些糖蛋白，采用二维等电聚焦/SDS-PAGE技术和LC-Q-TOF 技术正在使用。在分离和电印迹到PVDF膜上之后，标记的植物凝集素用于 鉴定印迹糖蛋白上独特的糖基化模式。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Expression of the human oncogene, her-2, causes selective increases in N-acetylglucosaminyltransferase T-V (GlcNAc T-V) mRNA and activity. Histological studies of breast tissue using a plant lectin that binds to the product of GlcNAc T-V show that the product is absent from non-diseased breast tissue, but is expressed when cells appear neoplastic. The GlcNAc T-V product increases in expression during tumor progression. This project focuses on identifying particular glycoproteins in breast biopsy tissue that express the GlcNAc T-V product in order to define possible biochemical tumor markers for breast carcinoma. To identify these glycoproteins, two-dimensional isoelectric focusing/SDS-PAGE techniques and LC-Q-TOF techniques are being used. After separation and electroblotting to PVDF membranes, labeled plant lectins are used to identify unique glycosylation patterns on the blotted glycoproteins.