Functional Genomics

功能基因组学核心（FGC）的目标是提供一个全面的，综合的方法， 癌症中心研究人员的基因组学研究。目前，FGC提供一系列微阵列和 数据分析服务。微阵列基因表达谱分析服务是基于Affyscore 基因芯片的寡核苷酸阵列平台。基本和高级数据分析由 生物统计学家和生物信息学家与生物医学信息学核心。FGC是 目前正在扩大提供两个新的主要服务，基于RNAi的技术和基因分型。 前者允许使用诸如siRNA的方法进行基因的功能分析，而后者提供了 中等通量SNP和DMA甲基化分析。所有的FGC服务都是由高度 经验丰富的核心经理，在其专业领域拥有广泛的专业知识。凝聚力和 FGC所有组成部分之间的协同作用由科学主任提供，他定期领导 与所有核心经理举行会议，讨论分析开发、数据分析、故障排除、集成， 和新技术。核心经理向研究者提供实验设计建议， 实验室和临床研究、试验开发和研究期间的质量控制以及 由核心生成的数据。这种“一站式”的方式使功能基因组学研究更加 癌症中心的研究人员可以广泛接触到。因此，FGC提供先进的技术， 支持加强功能基因组学研究的基础、临床和人口研究， 所有COHCCC项目的合作。在上一个12个月报告期内， 来自所有5个研究项目的14名癌症中心成员使用;同行评审的使用率占76% 总数的拟议的年度预算总额为339 788美元，其中39%为机构支助，18%为用户支助。 费用，16%其他，27%（91，000美元）要求CCSG。

The goal of the Functional Genomics Core (FGC) is to provide a comprehensive, integrated approach to genomics research for Cancer Center investigators. Presently, the FGC provides a range of Microarray and Data Analysis Services. The microarray gene expression profiling service is based on the Affymetrix oligonucleotide array platform of GeneChips. Basic and advanced data analysis is provided by biostatisticians and bioinformaticists in conjunction with the Biomedical Informatics Core. The FGC is currently undergoing expansion to provide two new major services, RNAi-based technology and genotyping. The former allows functional analyses of genes using approaches such as siRNA, and latter provides moderate-throughput SNP and DMA methylation analyses. All of the FGC services are provided by highly experienced Core Managers with extensive expertise in their areas of specialization. Cohesiveness and synergy among all of the FGC components are provided by the Scientific Director, who leads regular meetings with all Core Managers to discuss assay development, data analysis, trouble-shooting, integration, and new techniques. The Core Managers provide advice to investigators on experimental design prior to laboratory and clinical studies, assay development and quality control during the studies and interpretation of data generated by the Core. This "one-stop shopping" approach makes functional genomics research more broadly accessible to Cancer Center investigators. Thus, the FGC provides advanced technologies and support for basic, clinical and population studies that enhance functional genomics research and collaborations across all COHCCC programs. During the prior 12-month reporting period, the FGC was used by 14 Cancer Center members from all 5 Research Programs; peer-reviewed usage represented 76% of the total. The proposed total annual budget is $339,788, representing 39% institutional support, 18% user fees, 16% other, and 27% ($91,000) requested from the CCSG.