ENDOCRINOLOGY LABORATORY

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目及 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 在过去一年中，抗逆转录病毒技术中心为辅助生殖技术股以及坦桑尼亚联合共和国艾滋病方案审查中心的另外两个方案执行主任进行了区域影响评估。其中包括5项雌二醇测定和454项皮质醇水平测定。 RIA测定使用购自Diagnostic Products Corporation的试剂盒。这些试剂盒包括标准品、I125标记的激素和与特异性抗体结合的试管。 该测定是竞争性结合测定。基于结合至管的标记激素的量确定结果，并与标准品进行比较。在TNPRC开发的计算机程序用于建立“最佳拟合”曲线，并在测定后6至48小时内将结果提供给研究者，具体取决于测定的样品数量。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. In the past year the ART Core has conducted RIAs for the Assisted Reproductive Technology (ART) Unit as well as for two other PIs at the TNPRC. These included 5 assays for estradiol and 454 assays to determine cortisol levels. RIA assays utilized kits that were purchased from Diagnostic Products Corporation. These kits include standards, I125 labeled hormone, and tubes bound with specific antibody. The assay is a competitive binding assay. Results were determined based on the amount of labeled hormone bound to the tubes and compared to the standards. A computer program developed at the TNPRC was used to establish as "best fit" curve and results were provided to the investigators within 6 to 48 hours of the assay, depending on the number of samples assayed.