ASSISTED REPRODUCTIVE TECHNIQUES IN RHESUS MACAQUES

这个子项目是许多研究子项目中的一个 由NIH/NCRR资助的中心赠款提供的资源。子项目和 研究者（PI）可能从另一个NIH来源获得了主要资金， 因此可以在其他CRISP条目中表示。所列机构为 研究中心，而研究中心不一定是研究者所在的机构。 背景资料：通过使用从特定雄性收集的冷冻保存精子使合适的雌性授精，可以使控制繁殖产生特定基因型恒河猴的效率最大化。本研究旨在评估不同冷冻保存方案对猕猴精液解冻后存活率的影响。方法：收集5名成熟男性的多次射精，并分别用4种不同的方法冷冻。精液储存在液氮中，然后解冻。解冻后立即、30 min、60 min、240 min和18 h后再次评估总运动和前向运动。 结果：冷冻保存显着降低所有样本的运动，无论冷冻保存方法。结论：同一男性不同精液对两种方法的反应一致。然而，冷冻方法是决定即刻运动性的重要因素，尽管当所有样品的运动性几乎相同时，这种差异在4小时后消失。此外，个体雄性动物和冷冻方法之间存在显著的相互作用，这表明开发普遍适用的单一冷冻方案可能很困难。

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Backgrounds: The efficiency of controlled propagation to produce rhesus monkeys of particular genotypes can be maximized by use of cryopreserved spermatozoa collected from specific males to inseminate appropriate females. This study was designed to assess the effects of various cryopreservation protocols on the post-thaw survival of rhesus macaque semen. Methods: Multiple ejaculates of five mature males were collected and frozen by each of four different methods. Semen was stored in liquid nitrogen and then thawed. Total and progressive motility were assessed immediately after thawing, after 30 min, 60 min, 240 min and again 18 hours later. Results: Cryopreservation significantly reduced motility in all samples regardless of the cryopreservation method. Conclusion: The response of different ejaculates of the same male to each method proved to be consistent. However, the method of freezing was a significant factor in determining the immediate motility, although this difference had disappeared after four hours when motility was nearly the same in all samples. Moreover there was a significant interaction between individual males and freezing method suggesting that developing a single freezing protocol that is universally suitable may be difficult.