Agilent 6550 QTOF system for U of Utah

描述(由申请人提供)：犹他大学的代谢组学核心于2006年由NIH/NIDDK以分子血液学卓越中心的形式资助。从一开始，Core就使用气相色谱-质谱仪(GC-MS)对代谢物进行了非靶向性分析。核心最初专注于代谢中金属的关系，当关键金属代谢物过量或有限时，寻找疾病状态的根本原因。它将重点扩大到其他疾病，特别是那些由线粒体蛋白突变引起的疾病。核心小组在这方面的努力非常成功，由此产生的数据已列入若干出版物。并不是所有的代谢物都能用这种基于GC-MS的方法检测出来。在过去的一年里，Core使用了较旧的PE SCIEX API 365三重四极LC-MS/MS成功地执行了靶向代谢组学。不幸的是，这一仪器有很大的局限性；它较旧、不可靠，而且不能进行非目标分析。这种仪器需要被一种具有更高灵敏度、更大动态范围和更高可靠性的现代高端仪器所取代。我们建议购买一台安捷伦6550四极杆飞行时间(QTOF)质谱仪，配备安捷伦1290 Infinity超压液相色谱系统。该系统将补充我们目前的Waters GCT-Premier GC-MS，并将立即满负荷使用。我们目前正在进行一些与医学相关的代谢组学项目，从了解疾病原因的基础研究到实际的患者研究。

DESCRIPTION (provided by applicant): The Metabolomics Core at the University of Utah was initiated in 2006 with funding from NIH/NIDDK in the form of a Centers of Excellence in Molecular Hematology. From its inception the Core has performed non-targeted analysis of the metabolome using gas-chromatography based mass spectrometry (GC-MS). The Core initially focused on the relationship of metals in metabolism, searching for the underlying causes of disease states when key metal metabolites were either in excess or limiting. It has broadened its focus to other disease, especially those caused by mutations in mitochondrial proteins. The Core has been highly successful in this endeavor with resulting data has been included in a number of publications. Not all metabolites are detected using this GC-MS based approach. The Core over the past year has used an older PE SCIEX API 365 triple quadrupole LC-MS/MS to perform targeted metabolomics with success. Unfortunately this instrument suffers from major limitations; it is older, unreliable and does not perform non-targeted analysis. This instrument needs to be replaced with a modern, high-end instrument which has increased sensitivity, greater dynamic range and importantly improved reliability. We propose the purchase of an Agilent 6550 Quadrupole-Time-Of-Flight (QTOF) mass spectrometer with an Agilent 1290 Infinity ultrapressure liquid chromatography system. This system will complement our current Waters GCT-Premier GC-MS and will be used immediately at full capacity. We are pursuing a number of medically related metabolomics projects currently, from basic research in understanding the causes of disease to actual patient studies.