Core Grant for Vision Research

摘要： NEI视觉研究核心中心助学金的总体目标是增强视觉能力 通过促进轻松获得设备、培训和技术支持来研究科学家，这将扩大 实验能力，带来更具成本效益和时间效益的研究，并开启新的研究 方向。该中心将为德克萨斯大学西南医学中心的视觉科学研究人员提供服务 (UTSW)校园。它还将通过VISION促进新的研究合作和核心设施的使用 附近其他机构的科学家。 我们建议的NEI核心中心由三个资源/服务模块组成，每个模块由一名教师指导 在本单位的重点领域具有经验和专业知识的成员：1)细胞培养和细胞表型鉴定(指导 由Danielle Robertson博士编写)，它将支持维护和验证符合以下条件的细胞系 在广泛的研究活动中进行严格的研究所需的，并提供设备和支持 定量细胞分析应用；2)分子生物学/病毒生产(由John博士指导 Hulleman)，该公司将提供全方位的DNA生产和验证以及慢病毒和腺病毒 生产和提纯；以及，3)显微镜和数字成像(由Matthew Petroll博士指导)， 将提供设备、基础设施支持、用品和技术援助，以便对 细胞和组织。此外，该中心还包括一名生物统计学家，他将与调查人员合作 设计他们的研究报告，严格分析他们的数据，并解释他们的结果。 核心中心的具体目标是加强、简化和增加研究的科学严谨性 参与VISION科学家的活动：1)支持资源/服务模块的运行 通过管理使用和操作，并提供熟练的技术援助、设备维护、 和购买用品；2)向核心调查人员提供生物统计服务，以进行设计和 分析他们的研究成果；3)扩大合作研究项目的数量 UTSW内部以及UTSW与其他邻近机构之间的调查；4)创造更多 为初级教员的视觉研究项目的发展提供了肥沃的环境，并为新的 招聘到大学的视觉研究人员；以及，5)提供资源和培训，帮助毕业生 学生、医学生和博士后研究员与他们的研究。 总体而言，我们预计视力研究核心补助金将影响以下项目的研究计划 研究人员通过允许他们：1)通过增加新的实验扩展到新的研究领域 技术；2)与整个地区的其他核心中心用户发展新的协作；3)改善他们的 通过使用核心服务提高生产力和效率；以及，4)优化其实验设计和 分析以保持高度的严密性和重复性。

Abstract: The overall goal of this NEI Core Center Grant for Vision Research is to enhance the capabilities of vision research scientists by facilitating easy access to equipment, training and technical support which will expand experimental capabilities, result in more cost-effective and time-efficient research, and open up new research directions. This Center will serve vision science researchers across the UT Southwestern Medical Center (UTSW) campus. It will also facilitate new research collaborations and utilization of the core facilities by vision scientists at other nearby institutions. Our proposed NEI Core Center consists of three Resource/Service Modules, each directed by a faculty member with experience and expertise in their unit’s focus area: 1) Cell Culture and Cell Phenotyping (Directed by Dr. Danielle Robertson), which will support the maintenance and authentication of cell lines that are required for rigorous study across a wide range of research activities, and provide equipment and support for quantitative cellular analysis applications; 2) Molecular Biology/Virus Production (Directed by Dr. John Hulleman), which will provide full service DNA production and validation as well as lentivirus and adenovirus production and purification; and, 3) Microscopy and Digital Imaging (Directed by Dr. Matthew Petroll), which will provide equipment, infra-structural support, supplies and technical assistance for quantitative imaging of cells and tissues. In addition, the Center includes a biostatistician who will work with Investigators on the design of their research studies, rigorous analysis of their data, and interpretation of their results. The Specific Aims of the Core Center are to enhance, streamline and add scientific rigor to the research activities of the participating vision scientists by: 1) Supporting the operation of the Resource/Service Modules by managing usage and operations, and providing for skilled technical assistance, equipment maintenance, and the purchase of supplies; 2) providing biostatistical services to Core investigators for the design and analysis of their research studies; 3) expanding the number of collaborative research projects between investigators both within UTSW and between UTSW and other neighboring institutions; 4) creating a more fertile environment for the development of vision research programs of junior faculty members, and for new vision researchers recruited into the University; and, 5) providing resources and training to assist graduate students, medical students, and post-doctoral fellows with their research. Overall, we anticipate that the Core Grant for Vision Research will impact the research programs of these researchers by allowing them to: 1) expand into new research areas through the addition of novel experimental techniques; 2) develop new collaborations with other Core Center users throughout the region; 3) improve their productivity and efficiency through the use of Core services; and, 4) optimize their experimental design and analysis to maintain a high level of rigor and reproducibility.