Targeted Analysis Resource

项目摘要目标资源 目标分析资源是一个多学科、全方位服务的暴露生物标志物计划，旨在 将无机和有机环境措施纳入现有的NIH研究。我们每周举行一次指导会议 整个Lab Hub团队的委员会会议，以讨论工作、解决工作流程问题并确定优先级 资源和活动，以便为HHEAR客户提供最先进的服务。我们的团队已被 在将靶向暴露生物标志物的基础毒理学与研究相匹配方面具有相当的专业知识 设计、收集的生物样本类型和测量的健康表型。我们还考虑到 受试者年龄、职业和暴露情景。我们的实验室可行性评估有助于塑造 客户提交的更高影响力的研究。我们还建议客户防止使用珍贵的 被认为可能低于检测限（例如，测量有机磷酸盐）的分析样品 农药代谢物在郊区人口），根据我们多年的研究经验， 了解暴露情景。我们与行政核心合作协调服务，包括 样品管理、接收、贴标和跟踪。我们作为专家顾问与协调 中心、PI/客户和其他HHEAR资源。我们与数据中心协作， 成果报告，确保数据格式正确，有足够的元数据，并参与 暴露/表型关系的分析。我们使用自动化机器人样品处理， 制备方法、高通量分析程序和最先进的实验室间QA/QC 包括真实的时间色谱监测和数据库管理的程序， 先进的实验室信息管理系统（LIMS）软件。我们还自动化数据处理， 减少人为错误。我们的方法可确保正确的样品跟踪和数据报告，并避免 样本到结果由于有大量机构资源可供支配， 在过去三年中，将质谱仪的数量增加到14台，确保有能力最好地为 HHEAR网络的需求。我们的团队还进行了广泛的推广，是唯一的CHEMICAL实验室中心 组织国家暴露生物标志物研讨会（2018年11月2日至3日在西奈山举行，主题为 Exposomics），如管理核心中所述。QA/QC方案和标准操作程序， 所有目标化合物都已确定，以保持我们化学分析的严格性。我们开发 元素和有机化学品小组，总共评估200多种化学品。我们参加了所有 CHEMICAL授权金属和有机化学品的QA/QC和PT程序，以验证我们的精度， 准确性，并将为HHEAR这样做。总之，我们以高度的效率处理和分析样品 和准确性，提供专家科学咨询，并将我们在靶向暴露测定方面的工作与 其他集线器组件始终保持高度的生产力和成本效益。

Project Summary Targeted Resource The Targeted Analysis Resource is a multi-disciplinary, full service exposure biomarker program designed to integrate inorganic and organic environmental measures into existing NIH studies. We hold weekly Steering Committee meetings of the full Lab Hub team to discuss jobs, troubleshoot work flows, and prioritize resources and activities in order to provide state of the art service to HHEAR clients. Our team has considerable expertise in matching the underlying toxicology of targeted exposure biomarkers to the study design, to the biological specimen types collected, and to the health phenotypes measured. We also factor in subject age, occupation and the exposure scenarios. Our lab feasibility assessments help shape the science of a client's submission towards higher impact studies. We also counsel clients on preventing the use of precious samples on assays that are deemed likely to be below detection limits (e.g. measuring organophosphate pesticide metabolites in suburban populations) based on our many years of research experience and understanding of exposure scenarios. We work with the Administrative Core to coordinate services, including sample management, receiving, labeling and tracking. We serve as expert consultants with the Coordinating Center, PI/Clients and other HHEAR Resources. We collaborate with the Data Center in the management of results reporting, ensuring that data are properly formatted and have sufficient metadata, as well as participate in the analysis of exposure/phenotype relationships. We use automated robotic sample handling and preparation methods, high throughput analytical procedures and state of the art inter-laboratory QA/QC procedures which include real time chromatography monitoring and database management through state-of- the-art laboratory information management system (LIMS) software. We also automated data processing to reduce human error. Our methods ensure proper sample tracking and data reporting and avoid mismatches of samples to results. With substantial institutional resources at its disposal, the Targeted Resource has doubled the number of mass spectrometry instruments to 14 in the last 3 years, ensuring the capacity to best serve the needs of the HHEAR Network. Our team also conducts extensive outreach and was the only CHEAR lab hub to organize a National Exposure Biomarker Symposium (held Nov 2-3, 2018 at Mount Sinai and themed on Exposomics) as described in the Administrative Core. QA/QC protocols and standard operating procedures for all target compounds have been established to maintain the rigor of our chemical analyses. We developed panels for elements and organic chemicals that in sum assess more than 200 chemicals. We participated in all CHEAR mandated QA/QC and PT programs for metals and organic chemicals to verify our precision and accuracy and will do so for HHEAR. In sum, we process and analyze samples with a high degree of efficiency and accuracy, provide expert scientific consultation and integrate our work on targeted exposure assays with the other Hub components all the while maintaining a high degree of productivity and cost effectiveness.