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Development of a HT-ATACseq assay to identify epigenetic regulators of Ewing Sarcoma cancer cells (Chemical Biology Consortium/NCI Experimental Therapeutics Collaboration)

Development of a HT-ATACseq assay to identify epigenetic regulators of Ewing Sarcoma cancer cells (Chemical Biology Consortium/NCI Experimental Therapeutics Collaboration)
开发 HT-ATACseq 测定法来鉴定尤文肉瘤癌细胞的表观遗传调节因子（化学生物学联盟/NCI 实验治疗合作组织）
批准号：
10916055
负责人：
Mark Henderson
金额：
$12.58万
依托单位：
NATIONAL CENTER FOR ADVANCING TRANSLATIONAL SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/10916055
关键词：
Biological AssayBiologyCellsChemicalsChromatinCollaborationsDNADevelopmentEnzymesEpigenetic ProcessEwings sarcomaGoalsInvestigational TherapiesMeasuresNational Center for Advancing Translational SciencesPatternPreparationResearchcancer cellcostcost effectivehigh throughput screeningsmall moleculesmall molecule libraries

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中文摘要
翻译
在此期间，项目团队完成了高通量筛选平台的实验条件优化，该平台能够使用基于酶的方法测量细胞中染色质可及性的动态状态（Assay for Transpose-Accessible chromatin， ATAC）。该小组已经验证了一种ATAC DNA的粗制方法，该方法节省了大量成本并提高了产量。利用优化后的检测方法，利用以表观遗传学为重点的小分子文库进行了中试筛选，活性化合物正在进行进一步的表征。
英文摘要
During this period, the project team has completed optimization of experimental conditions of a high-throughput screening platform capable of measuring dynamic states of chromatin accessibility in cells using an enzyme-based approach (Assay for Transpose-Accessible Chromatin (ATAC)). The group has validated a crude preparation approach for the ATAC DNA that saves significant cost and increases throughput. Using the optimized assay, a pilot screen was performed using an epigenetic-focused small molecule library, and active compounds are undergoing further characterization.
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会议论文
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国内基金
海外基金
Journal of Integrative Plant Biology
  • 批准号：
    31024801
  • 项目类别：
    专项基金项目
  • 资助金额：
    24.0万元
  • 批准年份：
    2010
  • 负责人：
    贺萍
  • 依托单位：
    中国科学院植物研究所