Research spectrofluorometer and microplate readuer

荧光照明和测量是当今医学和生物科学中应用最迅速的技术，这一事实促进了更复杂的荧光仪器和大量荧光染料的发展。在现代生物学的许多应用中，荧光分光光度法现已成为测量大样本平均荧光的首选方法。该方法通常用于核酸(DNA和RNA)和蛋白质的定量和纯度、酶联免疫吸附试验、酶动力学、钙、pH、氯、钠、钾等可用荧光染料测定的离子。随着绿色荧光蛋白和珊瑚礁珊瑚荧光蛋白等鲜活的彩色荧光蛋白的出现，以及荧光染料与蛋白质结合的简便性，对分子和蛋白质功能的研究变得越来越容易。尽管研究所的大多数实验室现在都可以使用这些技术，因为它拥有现代化的实验室和设备。目前，该研究所有一台荧光微孔板阅读器。然而，该仪器用于激发和发射的过滤器数量有限，这对于酶动力学和PicoGreen DNA检测等许多应用都是不准确的。此外，每当出现新的荧光染料时，我们都需要购买昂贵的滤光片。如果获得批准，扩大可获得的扫描光谱荧光计不仅为新的研究人员和学生打开了大门，也为端点、动力学和光谱等新的应用打开了大门。提案中描述的许多不同项目说明了新项目的多样性，新设备将使这些项目变得可行，并表明新设备将得到广泛应用。

Fluorescence illumination and measurement is the most rapidly expanding technique employed today, both in the medical and biological sciences, a fact which has spurred the development of more sophisticated fluorescence instrumentation and numerous fluorescent dyes. Spectrofluorometry has now become the method of choice in many applications in modern biology to measure the average fluorescence in bulk samples. This method is commonly used for nucleic acids (DNA and RNA) and protein quantitation and purity, ELISA, enzyme kinetics, calcium, pH, chloride, sodium, potassium and other ions that are measurable by fluorescent dyes. The availability of living colors fluorescent proteins such as the GFP and reef coral fluorescent proteins, and the ease of conjugating fluorescent dyes to proteins, investigations into the functionality of molecules and proteins has become increasingly accessible. Although these techniques are now accessible to most laboratories in the Institute because of its modern laboratories and equipments. Currently, one fluorescent microplate reader is available at the institute. However, this instrument uses a limited number of filter sets for excitation and emission, which is not accurate for many applications such as enzyme kinetics and PicoGreen DNA detection. In addition, whenever a new fluorescence dye comes out we need to buy expensive filters. If granted, the expanded availability of the scanning specrtofluorometer opens the door not only to new researchers and students, but also to new applications such as Endpoint, kinetic and spectrum. The many different projects described in the proposal illustrate the diversity of new projects, which will become feasible with new equipment, and demonstrate that the new equipment will be extensively utilized.