Digital imaging capability for NSERC sponsored, milti-user core microscope facility

显微镜学经历了一场数字革命，迅速改变了科学发现的格局。在这项赠款中，要求为滑铁卢大学生物系B1-179室跨部门、多用户核心设施中的两台大量使用的显微镜配备配备现代计算机成像和软件的数字CCD相机。这些升级对于最大限度地利用核心基础设施是必要的，这些基础设施是十多年前通过成功的NSERC资助提供的透射电子显微镜（1991-1992年飞利浦CM10 100kV）和多发射，实时，光学显微镜（1994-1995年蔡司Axiovert 100荧光显微镜）。在这项拨款申请中，这个利用良好的显微镜设施的总基础设施价值的一小部分被要求（$125,222加元），以升级滑铁卢大学的这个核心设施，使其达到21世纪标准显微镜研究所必需的性能水平。数字成像能力将为滑铁卢大学的许多实验室提供科学研究机会：a)这一升级提高了滑铁卢大学5个系14个实验室所要求的充分利用显微镜的能力；b)购买拟议的数字CCD相机设备对滑铁卢大学实验室的研究潜力至关重要；c)数字成像能力将提高滑铁卢大学NSERC资助的显微镜的利用率和性能；d)购买数码CCD相机和超高速拍摄，将首次为滑铁卢大学带来实时、快速捕捉生物事件的机会；e)无障碍、维护良好的核心显微镜设备是滑铁卢大学独一无二的研究设备；f)核心显微镜被大量用于培养高素质人才。

Microscopy has undergone a digital revolution that has rapidly transformed the landscape for scientific discovery.  In this grant, digital CCD cameras accompanied with modern computer imaging and software are being requested for two heavily-used microscopes in a cross-departmental, multi-user core facility in Room B1-179, Department of Biology at the University of Waterloo.  These upgrades are necessary for maximizing the use of core infrastructure that was provided over a decade ago through successful NSERC grants for transmission electron microscopy (Philips CM10 100kV in 1991-1992) and multi-emission, real-time, light microscopy (Zeiss Axiovert 100 fluorescence microscope in 1994-1995).  In this grant application, a small fraction of the total infrastructure value of this well-utilized microscope facility is requested ($125,222 CAD), to upgrade this core facility at the University of Waterloo to a performance level that is necessary for standard microscopy research in the 21st Century. Digital imaging capabilities will provide scientific opportunities to many laboratories at the University of Waterloo: a) This upgrade provides enhanced capabilities for well-utilized microscopes, requested by 14 laboratories in five Departments at the University of Waterloo.; b) Purchase of the proposed digital CCD camera equipment is vital to research potential for University of Waterloo laboratories.; c) Digital imaging capabilities will increase the utilization and performance of the NSERC funded microscopes at the University of Waterloo.; d) Purchase of the digital CCD camera and ultra-fast capture will bring opportunities to capture real-time and fast biological events for the first time to the University of Waterloo.; e) Accessible, well maintained, core microscope facility is one-of-kind at the University of Waterloo for research.; f) Core microscopes are heavily used for training highly qualified personnel.