Benchtop ultracentrifuge for subcellular fractionation of biologicals

我们正在寻求资金收购贝克曼Optima(TM) MAX-XP台式超离心机，该离心机具有两个固定角度转子（MLA-130, TLA-100.3），一个摆动斗转子（MS-50）和一个近垂直转子（TLN-120），提供全范围的容量和离心力。通过适当的适配器，这些转子可用于各种管尺寸，允许超离心容量从0.8 mL到5ml，每管。每个转子最多可容纳四管，允许工作从0.8到20毫升的样品。Optima(TM) MAX-XP具有非常快的加速度（< 1分钟），实现离心力高达10e6 x g（取决于转子）。因此，旋转速度比标准的地板模型更快，这是小体积重组蛋白或细胞提取物的理想选择。Optima(TM) MAX-XP的多功能性在六位有兴趣常规使用它的申请人进行的各种研究中得到了证明。例如，Optima(TM) MAX-XP超离心机将用于从各种生物来源分离线粒体、微粒体、精子核和低丰度蛋白质。统一的方面是需要在相对较小的规模上常规地产生高度纯化的亚细胞成分。

We are seeking funds for the acquisition of a Beckman Optima(TM) MAX-XP bench top ultracentrifuge with two fixed angle rotors (MLA-130, TLA-100.3) one swinging bucket rotor (MS-50) and one near vertical rotor (TLN-120), providing a full range of capacity and centrifugal forces. With appropriate adapters, these rotors can be used for a variety of tube sizes, allowing ultracentrifugation of volumes from 0.8 mL up to 5 mL, per tube. Each rotor holds up to four tubes, allowing work up of from 0.8 to 20 mL of sample. The Optima(TM) MAX-XP has very rapid acceleration (< 1 min), achieving centrifugal forces up to 10e6 x g (depending on the rotor). Spins are therefore more rapid than a standard floor model, which is ideal for working with small volumes of recombinant protein or cell extracts. The versatility of the Optima(TM) MAX-XP is evident in the variety of research conducted by the six applicants interested in using it routinely. For example, the Optima(TM) MAX-XP ultracentrifuge will be used to isolate mitochondria, microsomes, sperm nuclei and low abundance proteins from a variety of biological sources. The unifying aspect is the need to routinely generate highly purified subcellular components on a relatively small scale.