Concentrated food systems for sustainable membrane-based fractionation processes

人们越来越关注工业食品加工的可持续性和环境影响，这与创造高价值食品成分的新挑战相抵触，同时使用更少的不可再生能源，更少的饮用水，以及通过反渗透（RO）最大限度地减少产品损失，以降低运输成本并减少下游加工所涉及的体积。虽然预浓缩降低了总体环境影响，但浓缩食品系统的物理化学和功能特性在很大程度上得到了改变。****我们研究计划的长期目标是了解和利用浓缩系统的特定物理化学特性，为复杂蛋白质混合物的分离开发新的处理方法。我们的假设是，膜分离可以用于设计浓缩物，其中不同的现象，如溶解度变化，蛋白质聚集，脂-蛋白相互作用和盐沉淀可以增强/控制，并用作分离辅助剂，以改善分馏过程。****1)表征高浓度体系中蛋白质溶解度、聚集和蛋白质-蛋白质相互作用等理化性质的变化。****2)确定促进蛋白质不稳定或某些乳清成分聚合并使其分离的物理化学条件；****3)确定浓缩-滤滤和滤滤模式的组合，以优化净化；****4)将乳清蛋白开发的策略应用于酪蛋白浓缩物，用于分离单个酪蛋白。****我们的研究项目将为改善乳制品原料的生产提供可持续的替代方案。通过结合基础研究和应用研究，该项目将为高素质人才提供独特的培训机会，他们将受益于STELA乳业研究中心独特的研究环境，并与乳业接触。******

The growing concern about sustainability and environmental impact of industrial food processing opposes new challenges to create high-value food ingredients while using less non-renewable energy, less drinking water, and also, minimizing products losses by reverse osmosis (RO) to decrease transportation costs and reduce the volumes involved in downstream processing. While pre-concentration decreases the overall environmental impact, physicochemical and functional properties of concentrated food systems are largely modified.****The long-term goal of our research program aims at understanding and using specific physicochemical characteristics of concentrated systems to develop new processing approaches for the fractionation of complex protein mixtures. It is our hypothesis that membrane separation can be used to design concentrates in which different phenomena such as solubility changes, protein aggregation, lipid-protein interactions and salts precipitation can be enhanced/controlled and used as separation aid to improve fractionation processes.****1) To characterize the changes in physicochemical properties such as protein solubility, aggregation and protein-protein interaction in highly-concentrated systems.****2) To identify the physicochemical conditions that enhance protein destabilization or polymerization of some of whey components and enables their separation;****3) To identify combinations of concentration-diafiltration and diafiltration modes to optimize purification;****4) Apply the strategy developed for whey proteins to casein concentrates for the separation of individual caseins.****Sustainable alternatives for improving the manufacture of dairy-based ingredients will be generated by our research program. By combining fundamental and applied research, this program will provide unique training opportunities for highly-qualified personnel who will benefit from a unique research environment at STELA Dairy research Center and contacts with the dairy industry.******