High resolution microscopy using a scattering layer

使用散射层的高分辨率显微镜

• 批准号: 326167230
• 负责人: Professor Dr. Wolfgang Osten
• 金额: --
• 依托单位: Institut für Technische Optik (ITO)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2017
• 资助国家: 德国
• 起止时间: 2016-12-31 至 2020-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/326167230?language=en
• 关键词: resolution; microscopy; using; scattering; layer

Microscope objectives having good imaging quality and high resolution are expensive and bulky. At the same time, fixed focal length, working distance, limited depth of focus and magnification restrict their applicability. Aberrations are an additional challenge of classical microscope objectives. Consequently, there is a growing need for compact optical microscopy imaging systems with adjustable depth of focus, high resolution and free of aberration. The goal of the project is the development of a technique for imaging microscopic samples, where instead of a microscope objective, like in conventional microscopy, a scattering surface is applied. The image of the object will be retrieved by processing the light diffracted by the diffuser, recorded on a pixelated sensor. The newly developed microscope will deliver submicrometer resolution and aberration free images. Furthermore, it will likewise be applicable to shorter (UV, X-rays) and longer (infrared) wavelengths. The developed technique will be used for biological imaging and investigation of technical samples.

具有良好成像质量和高分辨率的显微镜物镜是昂贵且笨重的。同时，固定的焦距、工作距离、有限的焦深和放大倍数限制了它们的适用性。像差是传统显微镜物镜的另一个挑战。因此，越来越需要具有可调焦深、高分辨率和无像差的紧凑型光学显微成像系统。该项目的目标是开发一种显微镜样品成像技术，其中应用散射表面代替传统显微镜中的显微镜物镜。物体的图像将通过处理漫射器衍射的光来检索，记录在像素化传感器上。新开发的显微镜将提供亚微米分辨率和无像差的图像。此外，它同样适用于较短（UV，X射线）和较长（红外）波长。开发的技术将用于生物成像和技术样品的调查。

项目成果

Scatter-plate microscopy with spatially coherent illumination and temporal scatter modulation.

• DOI: 10.1364/oe.412047
• 发表时间: 2021-01
• 期刊: Optics express
• 影响因子: 3.8
• 作者: S. Ludwig;Pavel Ruchka;G. Pedrini;Xiang Peng;W. Osten

Scatter-plate microscope: improved image acquisition

散射板显微镜：改进的图像采集

• DOI: 10.1117/12.2306252
• 发表时间: 2018
• 作者: Stephan Ludwig;Wolfgang Osten
• 通讯作者: Wolfgang Osten

Single-pixel scatter-plate microscopy.

• DOI: 10.1364/ol.420593
• 发表时间: 2021-05
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: S. Ludwig;G. Pedrini;Xiang Peng;W. Osten

Image reconstruction and enhancement by deconvolution in scatter-plate microscopy.

• DOI: 10.1364/oe.27.023049
• 发表时间: 2019-08
• 期刊: Optics express
• 影响因子: 3.8
• 作者: S. Ludwig;B. Le Teurnier;G. Pedrini;Xiang Peng;W. Osten

Ensemble cross-correlation for image retrieval from the intensity signal recorded by a single pixel

• DOI: 10.1117/12.2592727
• 发表时间: 2021-06
• 作者: S. Ludwig;G. Pedrini;Xiang Peng;W. Osten