Novel Classes of Photoresponsive DNA Aptamers

新型光响应 DNA 适配体

• 批准号: 426929456
• 负责人: Professor Dr. Michael Famulok
• 金额: --
• 依托单位: Abteilung für Chemische Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/426929456?language=en
• 关键词: Novel; Classes; Photoresponsive; DNA; Aptamers

The proposed project will attempt to select DNA aptamers for a selection of photochromes that possess exemplary photophysical properties both in ultra-violet and visible wavelengths. The photochromes chosen for this study have excellent thermal-reversionproperties so can be addressed exclusively with photonic energy. Currently, only few RNA-based aptamers are available for basic photochromic molecules and have not found further application. The advances in recent years towards the selection of selective, strong binding aptamers for small molecules and improved and novel photochromic elements suggest the time is ripe to select DNA aptamers for these selected molecules. We will use both capture SELEX and more traditional solid-support approaches in gaining our aptamers to give ourselves as much opportunity to gain good aptamers. After truncation and thorough characterisation, we will then apply them to our dsDNA nanoarchitectures to demonstrate their applicability in controlling intercomponent motion using exclusively different wavelengths of light. This will be the first time that aptamers have been employed as binding elements within DNA interlocked structures and will engender light-switchable DNA interlocked systems without the use of arguably wasteful toehold ROs composed of a number of AB nucleoside surrogates, most notably in the visible-light region. However, the DNA aptamers selected within the main body of this proposal will have a plethora of potential applications in other fields. Fields such as those of synthetic biology and optochemical genetics will be offered new avenues by combining the putative biological compatibility of DNA aptamers and the visible light wavelength-switching of modified and novel photochromic elements.

拟议的项目将试图选择DNA适体，用于选择在紫外线和可见光波长下都具有示范性光物理特性的光致变色剂。本研究选择的光致变色材料具有优异的热回复特性，因此可以专门用光子能量来解决。目前，只有少数基于RNA的适体可用于基本光致变色分子，并且没有发现进一步的应用。近年来在选择用于小分子的选择性强结合适体和改进的新颖的光致变色元件方面的进展表明，为这些选定的分子选择DNA适体的时机已经成熟。我们将使用捕获SELEX和更传统的固相支持方法来获得我们的适体，以使我们有尽可能多的机会获得好的适体。在截断和彻底的表征之后，我们将把它们应用到我们的dsDNA纳米结构中，以证明它们在使用完全不同波长的光控制组件间运动中的适用性。这将是适体首次被用作DNA互锁结构内的结合元件，并且将产生光可切换的DNA互锁系统，而不使用由许多AB核苷替代物组成的可以说是浪费的立足点RO，最值得注意的是在可见光区域。然而，在本提案的主体中选择的DNA适体将在其他领域具有大量的潜在应用。合成生物学和光化学遗传学等领域将通过结合DNA适体的假定生物相容性和改性的和新颖的光致变色元件的可见光波长切换而提供新的途径。