Novel Classes of Photoresponsive DNA Aptamers

新型光响应 DNA 适配体

• 批准号: 426929456
• 负责人: Professor Dr. Michael Famulok
• 金额: --
• 依托单位: Abteilung für Chemische Biologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2019
• 资助国家: 德国
• 起止时间: 2018-12-31 至 2021-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/426929456?language=en
• 关键词: Novel; Classes; Photoresponsive; DNA; Aptamers

The proposed project will attempt to select DNA aptamers for a selection of photochromes that possess exemplary photophysical properties both in ultra-violet and visible wavelengths. The photochromes chosen for this study have excellent thermal-reversionproperties so can be addressed exclusively with photonic energy. Currently, only few RNA-based aptamers are available for basic photochromic molecules and have not found further application. The advances in recent years towards the selection of selective, strong binding aptamers for small molecules and improved and novel photochromic elements suggest the time is ripe to select DNA aptamers for these selected molecules. We will use both capture SELEX and more traditional solid-support approaches in gaining our aptamers to give ourselves as much opportunity to gain good aptamers. After truncation and thorough characterisation, we will then apply them to our dsDNA nanoarchitectures to demonstrate their applicability in controlling intercomponent motion using exclusively different wavelengths of light. This will be the first time that aptamers have been employed as binding elements within DNA interlocked structures and will engender light-switchable DNA interlocked systems without the use of arguably wasteful toehold ROs composed of a number of AB nucleoside surrogates, most notably in the visible-light region. However, the DNA aptamers selected within the main body of this proposal will have a plethora of potential applications in other fields. Fields such as those of synthetic biology and optochemical genetics will be offered new avenues by combining the putative biological compatibility of DNA aptamers and the visible light wavelength-switching of modified and novel photochromic elements.

拟议的项目将尝试选择DNA适体用于选择具有典范的光物理特性的光色素和可见波长中的典范。为这项研究选择的光更色素具有出色的热逆转录，因此可以专门用光子能来解决。当前，仅用于基本的光致变色分子，尚未找到进一步的应用。近年来，用于选择小分子和改进和新颖的光致变色元素的选择性，强结合适体的进步表明，为这些选定分子选择DNA适体的时间已经成熟。我们将同时使用捕获SELEX和更传统的固体支持方法来获得适体，以赋予自己获得良好适体的机会。截断和彻底表征后，我们将它们应用于我们的dsDNA纳米结构，以证明它们在控制互动运动中使用独特的光的光长度的光。这将是第一次将适体作为DNA互锁结构内的结合元件，并且会导致可轻开的DNA互锁系统，而无需使用可以说的浪费的toehold toehold ros，该toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold toehold syperhold ros ros均由许多AB核苷替代物组成，最值得注意的是在可见光的区域中最值得注意。但是，该提案主体中选择的DNA适体将在其他领域具有大量潜在应用。通过将DNA适体的假定生物学兼容性和可见的光波长转换结合了修改和新颖的光子色素元素的假定生物学兼容性，将为合成生物学和光化学遗传学的领域提供新的途径。