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Localization and function of histamine H3 receptors in the nasal mucosa

鼻粘膜组胺 H3 受体的定位和功能

基本信息

批准号：
14571615
负责人：
TAKEUCHI Kazuhiko
金额：
$ 2.05万
依托单位：
Mie University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2003
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571615/
关键词：
nasal mucosa histamine histamine H3 receptors submucosal glands synapses mucus secretion 粘膜下腺鼻アレルギー RT-PCR

项目摘要

By RT-PCR, histamine H3 receptor mRNA was not expressed in the nasal mucosa in the inferior turbinate of allergic rhinitis patients. In order to clarify the localization of histamine H3 receptors in the nasal mucosa, immunohistochemical study was performed using a monoclonal antibody against histamine H3 receptor in the inferior turbinate of allergic rhinitis patients. Positive signals were observed around submucosal glands. The localization of the positive signals on the periphery of the gland suggests two possibilities. First, H3 receptors may be on the basolateral membranes of gland cells. Second, H3 receptors may be on the presynaptic region of the nerves innervating these glands cells. Absence of H3 receptor mRNA in the nasal tissues supports the latter possibility. The above results suggest a possible involvement of histamine H3 receptors in mucus secretion of submucosal gland. To examine this, we administered H3 receptor agonist α-methylhistamine ( 10mg/kg), histamine H3 receptor antagonist thioperamide (5mg/kg) or physiological saline intraperitoneally in rats and its effects on degranulation of submucosal glands were evaluated by Alcian blue/periodic acid-Schiff (AB/PAS) staining. Thioperamide significantly induced degranulation in the submucosal gland in the nasal septum compared with the methylhistamine group and the control group. These results suggest that histamine H3 receptors play some roles in mucus secretion in submucosal gland in the nose.

经RT-PCR检测，变应性鼻炎患者下鼻甲组织中未见组胺H3受体基因的表达。为了明确组胺H3受体在鼻黏膜中的定位，用抗组胺H3受体的单抗对变应性鼻炎患者下鼻甲进行了免疫组织化学研究。粘膜下腺周围可见阳性信号。腺体周围阳性信号的定位表明有两种可能性。首先，H3受体可能位于腺细胞的基侧膜上。其次，H3受体可能位于支配这些腺体细胞的神经的突触前区域。鼻部组织中H3受体基因的缺失支持后一种可能性。以上结果提示，组胺H3受体可能参与了粘膜下腺的粘液分泌。为研究H3受体激动剂α-甲基组胺(10 mg/kg)、组胺H3受体拮抗剂硫代巴比妥胺(5 mg/kg)或生理盐水对大鼠粘膜下腺脱颗粒的影响，用阿新蓝/高碘酸席夫(AB/PAS)染色观察其对粘膜下腺脱颗粒的影响。与甲基组胺组和对照组相比，硫代巴比妥钠显着诱导鼻中隔粘膜下腺脱颗粒。这些结果提示，组胺H3受体在鼻粘膜下腺的粘液分泌中起一定作用。