Functional analysis of equine herpesvirus-1 receptor and its application for infection prevention

从马脑微血管内皮细胞cDNA表达文库中克隆了马MHCI类分子A68，并对其作为EHV-1受体的功能进行了分析。研究表明，马MHC I类是一种功能性gD受体，在EHV-1进入马细胞中起关键作用，并且还表明MHC I类分子173位的氨基酸残基参与EHV-1进入的效率。此外，我们还建立了表达A68的转基因小鼠，并评估了可溶性A68分子预防EHV-1感染的有效性。

We analyzed the function of equine MHC class I molecule A68, which was cloned from an equine brain microvascular endothelial cell cDNA expression library as an EHV-1 receptor. Studies suggest that equine MHC class I is a functional gD receptor that plays a pivotal role in EHV-1 entry into equine cells, and also suggest that the amino acid residue at position 173 of the MHC class I molecule is involved in the efficiency of EHV-1 entry. In addition, we generated transgenic mice expressing A68, and also evaluated the usefulness of soluble forms of A68 molecules for preventing EHV-1 infection.