Antigenic analysis of Norovirus and its application for serological diagnosis

诺如病毒（NoV）是冠状病毒科的一员，是急性非细菌性胃肠炎的主要病因之一。由于缺乏NoV的培养系统，现在无法测量对NoV的中和抗体。在杆状病毒感染的昆虫细胞中，将不同抗原类型的病毒衣壳蛋白以病毒样颗粒（vlp）的形式表达，并利用vlp进行ELISA抗体检测。应用研制的抗体检测酶联免疫吸附试验（ELISA）进行血清流行病学研究和血清学诊断。

Norovirus (NoV), a member of the family Caliciviridae, is one of the major cause of acute non-bacterial gastroenteritis. Due to the lack of a culture system for NoV, measurement of neutralization antibody to NoV is now impossible. Capsid proteins of different antigenic types of NoV strains were expressed as virus-like particles (VLPs) in baculovirus-infected insect cells, and VLPs were used for detection of antibody to NoV by ELISA. The developed antibody-detection ELISA was applied for seroepidemiological study and serological diagnosis of NoV infection.