Metabolome analysis in the gastric carcinogenesis induced by the cross-talk between Helicobacter pylori and host.

测定了从日本分离的4株幽门螺杆菌的全基因组序列。一个连接明确的核心基因的系统发育树支持东亚血统与欧洲血统祖先的分化，然后是从美洲血统祖先的分化。系统发育分析显示，外膜蛋白的谱系有很大的差异。这些结果明确了幽门螺杆菌的东亚血统，并为了解其致病机理提供了必要的信息。我们利用液-质联用、气相色谱-质谱法对代谢组进行了分析。幽门螺杆菌感染的AGS细胞和胃癌患者血清中乳酸水平升高。相比之下，幽门螺杆菌感染的AGS细胞和胃癌患者血清中苹果酸的表达下调。

The complete genome sequences of four Helicobacter pylori strains isolated from Japan were determined. A phylogenetic tree of concatenated well-defined core genes supported divergence of the East Asian lineage from the European lineage ancestor, and then from the Amerind lineage ancestor. Phylogenetic profiling revealed a large difference in the repertoire of outer membrane proteins. The results define H. pylori East Asian lineages and provide essential information for understanding their pathogenesis.We investigated metabolome analysis using liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry. Lactic acid was upregulated in H. pylori infected AGS(gastric cancer cell line) cells and serum of gastric cancer patients. In contrast, malic acid was downregulated in H. pylori infected AGS cells and serum of gastric cancer patients.