Metabolome analysis in the gastric carcinogenesis induced by the cross-talk between Helicobacter pylori and host.

测定了日本分离的4株幽门螺杆菌的全基因组序列。一个系统发育树连接明确的核心基因支持东亚血统的分歧，从欧洲血统的祖先，然后从美洲血统的祖先。系统发育分析显示，在剧目的外膜蛋白的一个很大的差异。结果定义了H. pylori东亚谱系，并提供必要的信息，了解其发病机制。我们研究了代谢组学分析，液相色谱-质谱，气相色谱-质谱。乳酸在H. pylori感染的AGS（胃癌细胞系）细胞和胃癌患者血清。相比之下，苹果酸在H. pylori感染的AGS细胞和胃癌患者血清。

The complete genome sequences of four Helicobacter pylori strains isolated from Japan were determined. A phylogenetic tree of concatenated well-defined core genes supported divergence of the East Asian lineage from the European lineage ancestor, and then from the Amerind lineage ancestor. Phylogenetic profiling revealed a large difference in the repertoire of outer membrane proteins. The results define H. pylori East Asian lineages and provide essential information for understanding their pathogenesis.We investigated metabolome analysis using liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry. Lactic acid was upregulated in H. pylori infected AGS(gastric cancer cell line) cells and serum of gastric cancer patients. In contrast, malic acid was downregulated in H. pylori infected AGS cells and serum of gastric cancer patients.