Metabolome analysis in the gastric carcinogenesis induced by the cross-talk between Helicobacter pylori and host.

幽门螺杆菌与宿主相互作用诱发胃癌的代谢组学分析。

• 批准号: 21390228
• 负责人: AZUMA Takeshi
• 金额: $ 11.56万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21390228/
• 关键词: H. pylori; プロテオミクス解析; メタボロミクス解析; H.pylori

The complete genome sequences of four Helicobacter pylori strains isolated from Japan were determined. A phylogenetic tree of concatenated well-defined core genes supported divergence of the East Asian lineage from the European lineage ancestor, and then from the Amerind lineage ancestor. Phylogenetic profiling revealed a large difference in the repertoire of outer membrane proteins. The results define H. pylori East Asian lineages and provide essential information for understanding their pathogenesis.We investigated metabolome analysis using liquid chromatography-mass spectrometry, gas chromatography-mass spectrometry. Lactic acid was upregulated in H. pylori infected AGS(gastric cancer cell line) cells and serum of gastric cancer patients. In contrast, malic acid was downregulated in H. pylori infected AGS cells and serum of gastric cancer patients.

测定了从日本分离的4株幽门螺杆菌的全基因组序列。一个连接明确的核心基因的系统发育树支持东亚血统与欧洲血统祖先的分化，然后是从美洲血统祖先的分化。系统发育分析显示，外膜蛋白的谱系有很大的差异。这些结果明确了幽门螺杆菌的东亚血统，并为了解其致病机理提供了必要的信息。我们利用液-质联用、气相色谱-质谱法对代谢组进行了分析。幽门螺杆菌感染的AGS细胞和胃癌患者血清中乳酸水平升高。相比之下，幽门螺杆菌感染的AGS细胞和胃癌患者血清中苹果酸的表达下调。

项目成果

IFN-γ plays an essential role in the pathogenesis of gastric lymphoid follicles formation caused by Helicobacter suis infection

• DOI: 10.1111/j.1574-695x.2011.00823.x
• 发表时间: 2011-10-01
• 期刊: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
• 作者: Mimura, Takuya;Yoshida, Masaru;Azuma, Takeshi
• 通讯作者: Azuma, Takeshi

Birth and death of genes linked to chromosomal inversion

• DOI: 10.1073/pnas.1012579108
• 发表时间: 2011-01-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Furuta, Yoshikazu;Kawai, Mikihiko;Kobayashi, Ichizo
• 通讯作者: Kobayashi, Ichizo

Infection of less virulent Helicobacter pylori strains in asymptomatic healthy individuals in Thailand as a potential contributing factor to the Asian enigma

• DOI: 10.1016/j.micinf.2009.12.007
• 发表时间: 2010-03-01
• 期刊: MICROBES AND INFECTION
• 影响因子: 5.8
• 作者: Hirai, Itaru;Sasaki, Tadahiro;Wangroongsarb, Piyada
• 通讯作者: Wangroongsarb, Piyada

Enhanced expression of CCL20 in human Helicobacter pylori-associated gastritis.

• DOI: 10.1016/j.clim.2008.09.016
• 发表时间: 2009-03
• 期刊: Clinical immunology (Orlando, Fla.)
• 作者: Yoshida A;Isomoto H;Hisatsune J;Nakayama M;Nakashima Y;Matsushima K;Mizuta Y;Hayashi T;Yamaoka Y;Azuma T;Moss J;Hirayama T;Kohno S
• 通讯作者: Kohno S

Helicobacter suis KB1 derived from pig gastric lymphoid follicles induces the formation of gastric lymphoid follicles in mice through the activation of B cells and CD4 positive cells

• DOI: 10.1016/j.micinf.2011.02.007
• 发表时间: 2011-07-01
• 期刊: MICROBES AND INFECTION
• 影响因子: 5.8
• 作者: Yamamoto, Koji;Tanaka, Hiroshi;Azuma, Takeshi
• 通讯作者: Azuma, Takeshi