Analysis of ATBF1 expression in malignant mesothelioma with reference to its intracellular behavior and association with cancer stem cells

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We analyzed expression of ATBF1 protein in malignant mesothelioma and reactive mesothelial cells. Using anti-D1-120 antibody, ATBF1 expressed less frequently in the nuclei of malignant mesotheliomas, and significantly increased its expression in the cytoplasm. The cytoplasmic expression significantly correlated with the prognosis of malignant mesothelioma. Using anti-MB043 antibody, ATBF1 expressed with large dots in the nuclei of malignant mesothelial cells, whereas did not stained in the reactive mesothelial cells, thus MB043 is considered to be a useful marker for differentiating between malignant and benign mesothelial cells. The results indicate that expression of ATBF1 increases in malignant mesothelioma, but its localization probably changes in the tumor cells. ATBF1 may exist through the nucleus to the cytoplasm, or may be segmentized in the nucleus and the cytoplasm.