14 ERA-CAPS: Mechanistic Analysis of Quantitative Disease Resistance in Brassica by Associative Transcriptomics

14 ERA-CAPS：通过关联转录组学对芸苔属植物定量抗病性的机制分析

• 批准号: BB/N005007/1
• 负责人: Christopher Ridout
• 金额: $ 59.44万
• 依托单位: John Innes Centre
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2015
• 资助国家: 英国
• 起止时间: 2015 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FN005007%2F1
• 关键词: 14; ERA; CAPS; Mechanistic; Analysis

Brassica napus, a major world-wide crop, comprises a range of crop types including oilseed rape (OSR), grown for edible and industrial oil, biodiesel, protein for animal feed as well as leaf and root vegetables. Diseases are a major factor limiting production, a threat increasing due to climate change and the imminent withdrawal of agrochemicals in Europe. Improved disease control is an urgent priority and breeders are increasingly using quantitative disease resistance (QDR), which is considered broad-spectrum and durable.This research will identify the most useful QDR genes for OSR breeding and understand the mechanisms behind this to enable predictions of their effectiveness and durability. Our consortium combines the leading expertise on the major OSR pathogens, the latest research on defence mechanisms of resistance and expertise in association genetics to identify effective QDR genes. Our industrial partner, KWS, will provide expertise on deployment of QDR in the field and on the development of genetic markers for molecular breeding of improved OSR varieties.We will identify resistance to the most important pathogens of OSR: Sclerotinia sclerotiorum, Verticillium spp, Leptosphaeria maculans, Alternaria brassicicola, Pyrenopeziza brassicae, and the model pathogens Pseudomonas syringae and Botrytis cinerea. A panel of 192 diverse B. napus lines will be screened for resistance against these pathogens in controlled environments and at KWS field trial sites. Schools will contribute in a 'citizen science' project and evaluate resistance at locations throughout Europe. In the same lines, we will quantify induced defence responses to conserved pathogen-associated molecular patterns (PAMPs). We will also quantify salicylic acid, lignin, phenylpropanoid, glucosinolate, and indole metabolites that are implicated in resistance mechanisms. Using association transcriptomics, we will identify resistance gene loci against multiple pathogens and understand how this relates to metabolite production and PAMP-triggered immunity.To test hypotheses about their contribution to resistance, we include studies on specific genes. Whilst glucosinolates contribute to resistance they can reduce the quality of seed. GTR1 and GTR2 are transporters in Arabidopsis that control the allocation of glucosinolates to seeds. We will test gtr1 gtr2 mutants for fitness and create gtr TILLING mutants in Brassica rapa (B. napus A genome) to measure the glucosinolate partitioning between leaves and seed. The work could enable development of OSR with high leaf glucosinolate content for resistance, without compromising seed quality. We will introduce tomato receptor Ve1 into B. napus and assess its ability to mediate resistance against Verticillium wilt. This research will lead to more sustainable production of OSR, with higher productivity through lower vulnerability to biotic stress and less reliance on chemical inputs.

甘蓝型油菜（Brassica napus）是一种主要的世界性作物，包括一系列作物类型，包括油菜（OSR），种植用于食用和工业油、生物柴油、动物饲料蛋白以及叶和根类蔬菜。疾病是限制生产的一个主要因素，由于气候变化和欧洲即将取消农用化学品，这一威胁正在增加。提高病害控制是当务之急，育种者越来越多地使用被认为是广谱和持久的定量抗病性（QDR）。这项研究将确定OSR育种最有用的QDR基因，并了解其背后的机制，以便预测其有效性和持久性。我们的联盟结合了主要OSR病原体的领先专业知识，抗性防御机制的最新研究和关联遗传学的专业知识，以确定有效的QDR基因。我们的工业合作伙伴KWS将为QDR在田间的部署和OSR改良品种分子育种遗传标记的开发提供专业知识。我们将鉴定对OSR最重要病原体的抗性：核盘菌、轮枝菌、斑点细球腔菌、芸苔链格孢菌、芸苔核盘菌以及模式病原体假单胞菌和灰葡萄孢。一组192个不同的B。将在受控环境和KWS田间试验地点筛选油菜品系对这些病原体的抗性。学校将在一个“公民科学”项目中做出贡献，并评估欧洲各地的抵抗力。在同一行中，我们将量化保守的病原体相关分子模式（PAMPs）诱导的防御反应。我们还将量化水杨酸，木质素，苯丙素，芥子油苷，和吲哚代谢物，涉及耐药机制。使用关联转录组学，我们将识别针对多种病原体的抗性基因位点，并了解这与代谢产物产生和PAMP触发的免疫力的关系。为了验证关于它们对抗性的贡献的假设，我们包括对特定基因的研究。虽然芥子油苷有助于抵抗，但它们会降低种子的质量。GTR 1和GTR 2是拟南芥中控制芥子油苷向种子分配的转运蛋白。我们将在芜菁（B. napus A基因组）来测量叶和种子之间的硫代葡萄糖苷分配。这项工作可以使OSR的发展与高叶片硫代葡萄糖苷含量的抗性，而不影响种子质量。我们将番茄受体Ve 1导入B。并评估其介导抗黄萎病的能力。这项研究将导致更可持续的OSR生产，通过降低对生物胁迫的脆弱性和减少对化学投入的依赖来提高生产力。

项目成果

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape (Brassica napus)

• DOI: 10.1007/s00122-024-04569-1
• 发表时间: 2024-03-01
• 期刊: THEORETICAL AND APPLIED GENETICS
• 影响因子: 5.4
• 作者: Jacott，Catherine N.;Schoonbeek，Henk-jan;Wells，Rachel
• 通讯作者: Wells，Rachel

A complex receptor locus confers responsiveness to necrosis and ethylene-inducing like peptides in Brassica napus

• DOI: 10.1111/tpj.16760
• 发表时间: 2024-04-11
• 期刊: PLANT JOURNAL
• 影响因子: 7.2
• 作者: Yalcin，Hicret Asli;Jacott，Catherine N.;Wells，Rachel
• 通讯作者: Wells，Rachel

Pathogen lifestyle determines host genetic signature of quantitative disease resistance loci in oilseed rape ( Brassica napus )

病原体生活方式决定油菜（甘蓝型油菜）定量抗病位点的宿主遗传特征

• DOI: 10.1101/2023.08.02.551671
• 发表时间: 2023
• 作者: Jacott C

Novel gene loci associated with susceptibility or cryptic quantitative resistance to Pyrenopeziza brassicae in Brassica napus.

• DOI: 10.1007/s00122-023-04243-y
• 发表时间: 2023-03-23
• 期刊: THEORETICAL AND APPLIED GENETICS
• 影响因子: 5.4
• 作者: Fell, Heather;Ali, Ajisa Muthayil;Wells, Rachel;Mitrousia, Georgia K.;Woolfenden, Hugh;Schoonbeek, Henk-Jan;Fitt, Bruce D. L.;Ridout, Christopher J.;Stotz, Henrik U.
• 通讯作者: Stotz, Henrik U.

Necrosis and ethylene-inducing-like peptide patterns from crop pathogens induce differential responses within seven brassicaceous species.

作物病原体的坏死和乙烯诱导样肽模式在七种芸苔科植物中引起不同的反应。

• DOI: 10.1111/ppa.13615
• 发表时间: 2022-12
• 期刊: PLANT PATHOLOGY
• 影响因子: 2.7
• 作者: Schoonbeek, Henk-jan;Yalcin, Hicret Asli;Burns, Rachel;Taylor, Rachel Emma;Casey, Adam;Holt, Sam;Van den Ackerveken, Guido;Wells, Rachel;Ridout, Christopher J.
• 通讯作者: Ridout, Christopher J.