MECHANISMS OF OXYTOCIN-INDUCED MATERNAL BEHAVIOR

催产素引起的孕产行为的机制

• 批准号: 3313491
• 负责人: ARTHUR J. PRANGE
• 金额: $ 10.83万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-01-01 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3313491
• 关键词: arginine vasopressin; autoradiography; behavior test; brain metabolism; cerebrospinal fluid; dosage; experimental brain lesion; high performance liquid chromatography; hysterectomy; lactation; maternal behavior; neuropharmacology; ovariectomy; oxytocin; peptides; psychopharmacology; radioimmunoassay; statistics /biometry

We have reported that intracerebroventricular administration of oxytocin to virgin female rats induces a high incidence of rapid-onset full maternal behavior. The incidence of maternal behavior is dose related. Extensive specificity studies have shown that, in addition to oxytocin, arginine vasopressin and tocinoic acid (the ring structure of oxytocin) induce a lesser, though statistically significant, rate of full maternal behavior. Prostaglandin F2alpha induces transient partial maternal behavior. Many substances are inactive. The rapid induction of maternal behavior, full or partial, by active substances appears to be estrogen dependent. Thus our work is addressed to the triggers of maternal behavior as contrasted to the endocrine permission of such behavior. Our work, centered on peptides, complements earlier elegant descriptions of the role of steriod hormones. We propose to investigate the importance of brain release of endogenous oxytocin and arginine vasopressin th the onset of maternal behavior in parturient rats and to the maintenance of maternal behavior in nursing rats. We will assess the impact on onset and maintenance of maternal behavior of manipulations that deplete extra hypothalamic brain sites of oxytocin and vasopressin (lesioning of the paraventricular nucleus and/or the suprachiasmatic nucleus). For the same dependent variables we will assess the impact of manipulations that prevent oxytocin and vasopressin from interacting with receptors (intracerebroventricular injection of oxytocin- and vasopressin-directed antisera or infusion of competitive inhibitor analogs of these peptides). We propose to measure regional brain content and CSF and blood levels of oxytocin and arginine vasopressin and brain uptake of [3H] oxytocin in female rats in hormonal states either conducive or inimical to the onset of maternal behavior and in nursing and post nursing rats that have given birth. We intend to determing the loci at which oxytocin and arginine vasopressin have their maternal behavioral effects by injecting these peptides into selected brain sites.

我们报道了脑室内注射催产素 处女雌性大鼠诱发快速发病的全母性高发病率 行为。 母亲行为的发生率与剂量相关。 广泛的 特异性研究表明，除了催产素之外，精氨酸 加压素和生育酸（催产素的环状结构）诱导 尽管统计上显着，但完全母亲行为的比例较低。 前列腺素 F2α 诱导短暂的部分母性行为。 许多 物质不活跃。 快速诱导母性行为，完全或 部分，活性物质似乎是雌激素依赖性的。 因此我们的 工作针对的是母亲行为的触发因素，而不是针对母亲行为的触发因素。 内分泌允许这种行为。 我们的工作以肽为中心， 补充了早期对类固醇激素作用的优雅描述。 我们建议研究大脑释放内源性物质的重要性 催产素和精氨酸加压素对母亲行为的影响 分娩大鼠及护理中母性行为的维持 老鼠。 我们将评估对孕产妇发生和维持的影响 消耗下丘脑额外部位的操作行为 催产素和加压素（室旁核损伤和/或 视交叉上核）。 对于相同的因变量，我们将 评估预防催产素和加压素的操作的影响 与受体相互作用（脑室内注射 催产素和加压素导向的抗血清或竞争性输注 这些肽的抑制剂类似物）。 我们建议测量区域大脑 催产素和精氨酸加压素的含量和脑脊液以及血液水平 雌性大鼠在荷尔蒙状态下大脑对[3H]催产素的摄取 有助于或不利于母亲行为的发生以及护理和 哺乳已分娩的大鼠后。 我们打算确定基因座 催产素和精氨酸加压素在此时具有母体行为 通过将这些肽注射到选定的大脑部位来发挥作用。