NMR STUDIES OF CEREBRAL BLOOD FLOW AND ENERGY METABOLISM

脑血流和能量代谢的核磁共振研究

• 批准号: 3801923
• 负责人: A C MCLAUGHLIN
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3801923
• 关键词: bioenergetics; brain imaging /visualization /scanning; brain metabolism; cats; cerebrovascular disorder diagnosis; diagnosis design /evaluation; fluorohydrocarbon; microcapsule; noninvasive diagnosis; nuclear magnetic resonance spectroscopy

This study was undertaken to assess a new method for the non-invasive determination of cerebral blood flow without the use of radioactive tracers. Specifically, we used a new 19F NMR technique to measure the clearance of a fluorinated inert gas, CHF3, from the brain. We tested the new technique by comparing the cerebral blood flow values determined by 19F NMR with cerebral blood flow values determined simultaneously by radioactive microsphere techniques. The cerebral blood flow values determined by 19F NMR and radioactive microsphere techniques agreed reasonably well, and showed the same response to variations in the arterial CO2 level. We conclude that the 19F NMR technique gives a quantitative measure of cerebral blood flow. We used NMR techniques to localize the 19F NMR spectra (and thus the blood flow) to a well defined 4 cc volume in the brain. Using this localization technique, the 19F NMR approach was now non-evasive, and thus applicable to clinical studies. However, there were two drawbacks to this technique. First, the spatial resolution was rather poor, and second, only one region of the brain was investigated at a time. Both these problems were circumvented by a new technique that was developed in the last year. Instead of obtaining spectra from a single localized region, we used NMR techniques to image the cerebral blood flow over the entire brain. The spatial resolution for the imaging studies (0.8 cc) is much better than the spatial resolution obtained with the previous technique (4 cc).

这项研究是为了评估一种新的方法， 不使用放射性物质测定脑血流量 追踪器 具体地说，我们使用了一种新的19F NMR技术来测量 从大脑中清除氟化惰性气体CHF 3。 我们测试 通过比较脑血流值的新技术 通过19F NMR测定脑血流量值， 同时通过放射性微球技术。 脑 通过19F NMR和放射性微球测定的血流量值 技术同意相当不错，并表现出相同的反应， 动脉二氧化碳水平的变化。 我们得出结论，19F NMR 这项技术可以定量测量脑血流量。 我们使用NMR技术来定位19F NMR光谱（因此， 血流）到脑中明确定义的4cc体积。 使用此 定位技术，19F NMR方法现在是非回避的， 因此适用于临床研究。 然而， to this technology技术. 首先，空间分辨率相当差， 其次，每次只研究大脑的一个区域。 这两个问题都被一种新技术所规避， 去年开发的。 而不是从一个单一的光谱 局部区域，我们使用核磁共振技术来成像脑血 流经整个大脑 成像的空间分辨率 研究（0.8 cc）的空间分辨率比使用 前一种技术（4cc）。