IN VIVO NMR EXERCISE STUDIES IN AZT-RELATED MYOPATHY IN HIV+ PATIENTS

长期的AZT治疗可引起肌病，如光和 肌肉活检的电子显微镜。31P核磁共振波谱是一种 建立了研究肌病的方法，并可能对 在AZT治疗期间监测肌肉能量代谢。我们用了31P核磁共振 20例HIV阳性患者和19例年龄匹配患者的光谱分析 志愿者。接受AZT治疗的HIV阳性患者显示 显著更大程度的磷酸肌酸(PCR)耗竭 在给定工作负荷的情况下，与对照组相比。然而，被感染的艾滋病毒患者 与未接受AZT治疗的患者相比，未接受AZT治疗的患者没有显著的PCR缺失 控制。我们的发现支持以前的形态和分子 在接受AZT治疗的患者中观察到的肌病是由于 线粒体功能障碍。他们还证明了31P核磁共振 光谱学在检测方面与电子显微镜一样灵敏 AZT治疗患者的线粒体异常。我们得出的结论是31便士 核磁共振波谱是一种有用的非侵入性方法来监测艾滋病毒- AZT治疗期间的感染患者。

Long-term AZT therapy can cause myopathy, as demonstrated by light and electron microscopy of muscle biopsies. 31P NMR spectroscopy is an established method for studying myopathies, and could be helpful in monitoring muscle energy metabolism, during AZT therapy. We used 31P NMR spectroscopy to study 20 HIV positive patients and 19 age-matched volunteers. HIV-positive patients undergoing AZT therapy showed a significantly greater degree of phosphocreatine (PCr) depletion for a given work load, compared to controls. However, HIV patients that were not treated with AZT did not have a significant PCr depletion, compared to controls. Our findings support previous morphological and molecular finding that myopathy observed in AZT-treated patients is due to mitochondrial dysfunction. They also demonstrate that 31P NMR spectroscopy is as sensitive as electron microscopy in detecting mitochondrial abnormalities in AZT treated patients. We conclude that 31P NMR spectroscopy is a useful non-invasive approach for monitoring HIV- infected patients during AZT therapy.