IN VIVO NMR EXERCISE STUDIES OF HIV POSITIVE PATIENTS

HIV 阳性患者体内 NMR 运动研究

• 批准号: 3767548
• 负责人: A C MCLAUGHLIN
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3767548
• 关键词: AIDS therapy; HIV infections; creatine phosphate; drug adverse effect; hepatitis B; hepatitis B virus group; human immunodeficiency virus 1; liver disorder chemotherapy; mitochondria; muscle disorder diagnosis; muscle disorders; muscle metabolism; noninvasive diagnosis; nuclear magnetic resonance spectroscopy; pediatric AIDS; zidovudine

Long-term AZT therapy can cause myopathy, as demonstrated by light and electron microscopy of muscle biopsies. 31P NMR spectroscopy is an established method for studying myopathies, and could be helpful in monitoring muscle energy metabolism during AZT therapy. In the previous year, we used 31P NMR spectroscopy to study 20 HIV positive patients and 19 age-matched volunteers. HIV-positive patients undergoing AZT therapy showed a significantly greater degree of phosphocreatine (PCr) depletion for a given work load, compared to controls. However, HIV patients that were not treated with AZT did not have a significant PCr depletion, compared to controls. Our findings support previous morphological and molecular finding that myopathy observed in AZT-treated patients is due to mitochondrial dysfunction. They also demonstrate that 31P NMR spectroscopy is as sensitive as electron microscopy in detecting mitochondrial abnormalities in AZT-treated patients. We conclude that 31P NMR spectroscopy is a useful non-invasive approach for monitoring HIV- infected patients during AZT therapy. These studies have now been extended to investigate pediatric AIDS patients, and to investigate other cases where experimental drugs can cause mitochondrial damage. In particular, we have studied a number of patients that have been treated for hepatitis B with fialuridine.

长期AZT治疗可引起肌病，如光和 肌肉活检的电子显微镜检查。 31 P NMR光谱是一种 建立了研究肌病的方法，并可能有助于 在AZT治疗期间监测肌肉能量代谢 上一 年，我们使用31 P NMR光谱研究了20名HIV阳性患者， 19名年龄匹配的志愿者。 接受AZT治疗的艾滋病毒阳性患者 磷酸肌酸（PCr）消耗程度明显更高， 对于给定的工作负荷，与对照组相比。 然而，艾滋病患者， 未接受AZT治疗的患者没有明显的PCr耗竭， 与对照相比。 我们的研究结果支持以前的形态学和 在AZT治疗的患者中观察到的肌病是由于 线粒体功能障碍 他们还证明，31 P NMR 光谱学和电子显微镜一样灵敏， AZT治疗患者的线粒体异常。 我们得出结论，31 P 核磁共振波谱是一种有用的非侵入性方法，用于监测艾滋病毒- 在AZT治疗期间感染的病人 这些研究现在已经 扩展到调查儿科艾滋病患者，并调查其他 实验性药物可能导致线粒体损伤的病例。 在 特别是，我们研究了一些接受过治疗的患者， 治疗B型肝炎