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IN VIVO MEASUREMENT OF INTRACELLULAR CALCIUM IN THE BRAIN

IN VIVO MEASUREMENT OF INTRACELLULAR CALCIUM IN THE BRAIN
脑内细胞内钙的体内测量
批准号：
3801935
负责人：
A C MCLAUGHLIN
金额：
$0.0万
依托单位：
NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/3801935
关键词：
brain metabolism calcium metabolism calcium transporting ATPase fluorescent dye /probe intracellular transport

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中文摘要
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英文摘要
We have used calcium-sensitive fluorescent dyes to measure changes in intra cellular calcium concentrations in the intact brain. Changes in intracellular calcium determined with this approach can be correlated with changes in extracellular sodium levels determined simultaneously with ion-sensitive electrodes. Preliminary results show that in hypoxia changes in extracellular sodium levels occur well before changes in intracellular calcium are observed. Studies with perfused liver show that the dyes used for intracellular calcium determination leak out of the liver. The fluorescence can therefore not be used in the liver.
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