IN VIVO MEASUREMENT OF INTRACELLULAR CALCIUM IN THE BRAIN

脑内细胞内钙的体内测量

• 批准号: 3801935
• 负责人: A C MCLAUGHLIN
• 金额: --
• 依托单位: NATIONAL INSTITUTE ON ALCOHOL ABUSE AND ALCOHOLISM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3801935
• 关键词: brain metabolism; calcium metabolism; calcium transporting ATPase; fluorescent dye /probe; intracellular transport

We have used calcium-sensitive fluorescent dyes to measure changes in intra cellular calcium concentrations in the intact brain. Changes in intracellular calcium determined with this approach can be correlated with changes in extracellular sodium levels determined simultaneously with ion-sensitive electrodes. Preliminary results show that in hypoxia changes in extracellular sodium levels occur well before changes in intracellular calcium are observed. Studies with perfused liver show that the dyes used for intracellular calcium determination leak out of the liver. The fluorescence can therefore not be used in the liver.

我们使用钙敏感的荧光染料来测量 完整大脑中的细胞内钙浓度。 变化 用这种方法测定的细胞内钙可以与 同时测定细胞外钠水平的变化 离子敏感电极。 初步结果显示，在低氧条件下， 细胞外钠水平的变化早于 观察到细胞内钙。 灌注肝脏的研究表明，用于细胞内的染料， 钙测定从肝中漏出。 荧光可以 因此不能用于肝脏。