Conditional knockout of the murine patched gene for the study of skin differentiation and cancer.

条件性敲除小鼠补丁基因，用于皮肤分化和癌症的研究。

• 批准号: nhmrc : 143073
• 负责人: Prof Brandon Wainwright
• 金额: $ 28.24万
• 依托单位国家: 澳大利亚
• 项目类别: NHMRC Project Grants
• 财政年份: 2001
• 资助国家: 澳大利亚
• 起止时间: 2001-01-01 至 2003-12-31
• 项目状态: 已结题
• 来源: https://researchdata.edu.au/conditional-knockout-murine-differentiation-cancer/95212
• 关键词: Conditional; knockout; murine; patched; gene

Basal cell carcinoma (BCC) is the most common cancer in Australia. We recently isolated the BCC gene, Patched (PTCH) from analysis of patients with Naevoid Basal Cell Carcinoma Syndrome (NBCCS). Individuals with NBCCS have a wide variety of developmental defects in addition to a cancer predisposition which includes medulloblastoma, rhabdomyosarcoma and ovarian fibroma as well as multiple BCCs. This application proposes the generation of an animal model for skin development and cancer by selectively removing patched gene function from specific cell of the skin. In doing this we will be able to determine the exact role of this gene in skin development, and how mutation causes common skin cancer.

基底细胞癌（BCC）是澳大利亚最常见的癌症。我们最近从Naevoid基底细胞癌综合征（NBCCS）患者的分析中分离出BCC基因补丁（PTCH）。NBCCS患者除了易患癌症外，还存在多种发育缺陷，包括髓母细胞瘤、横纹肌肉瘤、卵巢纤维瘤以及多种BCCs。该应用程序提出了通过选择性地从皮肤的特定细胞中去除补丁基因功能来产生皮肤发育和癌症的动物模型。通过这样做，我们将能够确定该基因在皮肤发育中的确切作用，以及突变如何导致常见的皮肤癌。