CELLULAR PATHWAYS INVOLVED IN VIRAL INDUCTION--THEIR ROLE IN HIV PATHOGENESIS

使用U1细胞系研究了HIV从潜伏状态的诱导。 具体而言，各种化学致癌物和 检测了环境诱变剂诱导病毒的能力 从这个细胞系的目的是确定风险因素， 可能影响艾滋病疾病进展的生理相关性。 U1 细胞用2种不同浓度的各种 致癌物质。 通过p24抗原、RT、DNA和PCR检测病毒产生。 RNA分析。增强病毒生产中看到的细胞处理， 苯并芘、NNK和黄曲霉毒素B。 病毒诱导伴随着 增加NF-κ B活性和细胞因子如TNF-α的产生， α的 在黄曲霉毒素的情况下，病毒诱导似乎是TNF-α， 阿尔法独立机制 其他细胞因子的作用，第二 信使和细胞信号传导途径在诱导艾滋病毒， 这些特工目前正在接受调查。 这些实验 也在慢性感染细胞中进行， 病毒水平。

Induction of HIV from a latent state was studied using the U1 cell line. Specifically, the effect of various chemical carcinigens and environmental mutagens was examined for their ability to induce virus from this cell line with the objective of identifying risk factors of physiologic relevance that may affect disease progression in AIDS. U1 cells were treated with 2 different concentrations of various carcinogens. Virus production was measured by p24 antigen, RT, DNA and RNA analysis. Enhances virus production was seen in cells treated with benzopyrene, NNK and aflatoxin B. Viral induction was accompanied by an increase in NF- KB activity and production of cytokines such as TNF- alpha. In the case of aflatoxin, virus induction appeared to be a TNF- alpha independent mechanism. The role of other cytokines, second messengers and cellular signaling pathways in the induction of HIV by these agents is currently under investigation. These experiments are also being performed in chronically infected cells that produce low levels of virus.